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MiR-539 functions as a tumor suppressor in pancreatic cancer by targeting TWIST1
- Source :
- Experimental and Molecular Pathology. 108:143-149
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- The dysregulation of microRNA (miRNA) expression has been highlighted in a variety of human malignant conditions with reports implicating a critical role in the process of tumor growth. The role of miR-539 in pancreatic cancer (PC) is yet to be fully elucidated, hence the aim of the current study was to investigate the effect of miR-539 expression in relation to a cohort of 52 PC specimens. The application of a real-time quantitative polymerase chain reaction (qRT-PCR) revealed a significantly down-regulated miR-539 level, which was accompanied by an increased TWIST1 expression in PC when compared with the controls. The in vitro experiment results demonstrated that the endogenic mimic of miR-539 significantly suppressed the growth of the xenograft tumors in PANC-1 cells, when compared to the delivery of the control miRNA and blank control. Meanwhile, the key epithelial-mesenchymal transition (EMT) inducer, TWIST1 was verified as a direct target gene of miR-539 through the application of a luciferase reporter assay. In conclusion, the results of the current study present evidence emphasizing the significance of the interactions between miR-539 and TWIST1 in the development of and progression of PC, highlighting its potential as a therapeutic target in the treatment of PC patients.
- Subjects :
- Adult
Male
0301 basic medicine
Epithelial-Mesenchymal Transition
Clinical Biochemistry
Mice, Nude
Apoptosis
Biology
Pathology and Forensic Medicine
law.invention
Mice
03 medical and health sciences
0302 clinical medicine
Cell Movement
law
Cell Line, Tumor
Pancreatic cancer
microRNA
medicine
Animals
Humans
Inducer
Epithelial–mesenchymal transition
Molecular Biology
Aged
Cell Proliferation
Transition (genetics)
Twist-Related Protein 1
Nuclear Proteins
Middle Aged
In vitro experiment
medicine.disease
Xenograft Model Antitumor Assays
Pancreatic Neoplasms
MicroRNAs
030104 developmental biology
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
Cancer research
Suppressor
Female
Transcriptome
Subjects
Details
- ISSN :
- 00144800
- Volume :
- 108
- Database :
- OpenAIRE
- Journal :
- Experimental and Molecular Pathology
- Accession number :
- edsair.doi.dedup.....087136312e5ca4f37b5e8a19e1f4069b