Additional file 1 of Acquisition of cancer stem cell capacities after spontaneous cell fusion

Additional file 1: Figure S1. A. Cytometry dot plot. ALDHlow cells in blue and ALDHhigh cells in green. Top: cell line incubated with DEAB as negative control. Bottom: cell line without DEAB. B. Evaluation of stem cell frequency by ELDA method in ALDHlow and ALDHhigh cells after cell sorting. “-“: absence of CS/ICs. Figure S2. Percentage of ALDHhigh cells evaluated in parental and hybrid cell population after 72 h of coculture between E6E7 and RST (n = 17). Percentages were not statistically different. Figure S3. A. Cytometry dot plot representing cell populations selected for cell sorting. B. Images of spheres developed in non-adherent cell culture conditions; scale bar = 50 μm. Figure S4. Full length blots. Figure S5. Cytometry dot plot with gates used to determine percentage of fused cells after 72 h of co-culture. x-axis represents CFP fluorescence intensity and y-axis represents DsRed fluorescence intensity. Figure S6. Full length blots.