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Distinct vitellogenin domains differentially regulate immunological outcomes in invertebrates
- Source :
- The Journal of Biological Chemistry
- Publication Year :
- 2020
- Publisher :
- American Society for Biochemistry and Molecular Biology, 2020.
-
Abstract
- The classical role of Vitellogenin (Vg) is providing energy reserves for developing embryos, but its roles appear to extend beyond this nutritional function, and its importance in host immune defense is garnering increasing research attention. However, Vg-regulated immunological functions are dependent on three different domains within different species and remain poorly understood. In the present study, we confirmed three conserved VG domains—LPD_N, DUF1943, and VWD—in the Chinese mitten crab (Eriocheir sinensis), highlighting functional similarities of Vg in vertebrates and invertebrates. Of these three domains, DUF1943 and VWD showed definitive bacterial binding activity via interaction with the signature components on microbial surfaces, but this activity was not exhibited by the LPD_N domain. Antibacterial assays indicated that only the VWD domain inhibits bacterial proliferation, and this function may be conserved between different species due to the conserved amino acid residues. To further explore the relationship between Vg and polymeric immunoglobulin receptor (pIgR), we expressed EspIgR and the three E. sinensis Vg (EsVg) domains in HEK293T cells, and coimmunoprecipitation assay demonstrated that only the DUF1943 domain interacts with EspIgR. Subsequent experiments demonstrated that EsVg regulates hemocyte phagocytosis by binding with EspIgR through the DUF1943 domain, thus promoting bacterial clearance and protecting the host from bacterial infection. To the best of our knowledge, our work is the first to report distinct domains in Vg inducing different immunological outcomes in invertebrates, providing new evidence that pIgR acts as a phagocytic receptor for Vg.
- Subjects :
- 0301 basic medicine
EspIgR, E. sinensis polymeric immunoglobulin receptor
Hemocytes
Von Willebrand factor type D domain
invertebrate immunology
Biochemistry
Bacterial Adhesion
Vitellogenins
Crustacea
Phylogeny
NJ, neighbor-joining
polymeric immunoglobulin receptor (pIgR)
Cell biology
LB, Luria–Bertani
Research Article
hemocyte
Protein domain
pIg, polyimmunoglobulin
Biology
03 medical and health sciences
Vitellogenin
ORF, open reading frame
Phagocytosis
Protein Domains
pIgR, polymeric immunoglobulin receptor
vitellogenin (Vg)
Animals
Humans
CPZ, chlorpromazine
Amino Acid Sequence
Molecular Biology
Vg, vitellogenin
Innate immune system
030102 biochemistry & molecular biology
Bacteria
Base Sequence
HEK 293 cells
VWD, von Willebrand factor type D domain
bacterial infection
Cell Biology
TBS, Tris-buffered saline
Open reading frame
030104 developmental biology
HEK293 Cells
biology.protein
LLTP, large lipid transfer protein
SC, secretory component
Polymeric immunoglobulin receptor
IPTG, isopropyl b-D-1-thiogalactopyranoside
Function (biology)
Subjects
Details
- Language :
- English
- ISSN :
- 1083351X and 00219258
- Volume :
- 296
- Database :
- OpenAIRE
- Journal :
- The Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....086b69112c835784b4a8c06d735ed793