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Spike vs nucleocapsid SARS-CoV-2 antigen detection: application in nasopharyngeal swab specimens
- Source :
- Analytical and Bioanalytical Chemistry
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Public health experts emphasize the need for quick, point-of-care SARS-CoV-2 detection as an effective strategy for controlling virus spread. To this end, many “antigen” detection devices were developed and commercialized. These devices are mostly based on detecting SARS-CoV-2’s nucleocapsid protein. Recently, alerts issued by both the FDA and the CDC raised concerns regarding the devices’ tendency to exhibit false positive results. In this work we developed a novel alternative spike-based antigen assay, comprised of four high-affinity, specific monoclonal antibodies, directed against different epitopes on the spike’s S1 subunit. The assay’s performance was evaluated for COVID-19 detection from nasopharyngeal swabs, compared to an in-house nucleocapsid-based assay, composed of antibodies directed against the nucleocapsid. Detection of COVID-19 was carried out in a cohort of 284 qRT-PCR positive and negative nasopharyngeal swab samples. The time resolved fluorescence (TRF) ELISA spike-assay displayed very high specificity (99%) accompanied with a somewhat lower sensitivity (66% for CtAbstract FigureGraphic abstractSchematic representation of sample collection and analysis. The figure was created using BioRender.com
- Subjects :
- Coronavirus disease 2019 (COVID-19)
medicine.drug_class
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Enzyme-Linked Immunosorbent Assay
Spike
02 engineering and technology
Monoclonal antibody
Sensitivity and Specificity
01 natural sciences
Biochemistry
Epitope
Virus
COVID-19 Serological Testing
Specimen Handling
Analytical Chemistry
Antigen
TRF-ELISA
medicine
Coronavirus Nucleocapsid Proteins
Humans
Nasopharyngeal swab specimens
Nucleocapsid
biology
SARS-CoV-2
business.industry
010401 analytical chemistry
COVID-19
Phosphoproteins
021001 nanoscience & nanotechnology
Virology
0104 chemical sciences
Spike Glycoprotein, Coronavirus
biology.protein
Spike (software development)
Sample collection
Antibody
0210 nano-technology
business
Research Paper
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 413
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....0866cdee59976677d39b90b0c6bfc6de