Back to Search
Start Over
Make it or break it: Plant AEPs on stage in biotechnology
- Source :
- Biotechnology advances. 45
- Publication Year :
- 2020
-
Abstract
- Asparaginyl endopeptidases (AEPs) are cysteine proteases that control a myriad of cellular functions in plants, including maturation of seed storage proteins and programmed cell death. Recently, several noteworthy AEPs have been discovered that primarily function as transpeptidases rather than hydrolases, to instead catalyse the formation of new peptide bonds. These AEPs appear to have evolved for the cyclisation of a large class of plant defence peptides called cyclotides. Here we describe recent insights into the structural differences between AEPs that preference peptide ligation over hydrolysis. This knowledge is instrumental for the deployment of AEP ligases as biotechnological tools for in vitro applications such as protein labelling and or cyclization, and for plant molecular farming applications.
- Subjects :
- 0106 biological sciences
chemistry.chemical_classification
Large class
0303 health sciences
Proteases
Cellular functions
Bioengineering
Peptide
Computational biology
Biology
Plants
01 natural sciences
Applied Microbiology and Biotechnology
Cyclotide
Ligases
03 medical and health sciences
chemistry
Cyclization
010608 biotechnology
Cyclotides
Function (biology)
030304 developmental biology
Cysteine
Biotechnology
Plant Proteins
Subjects
Details
- ISSN :
- 18731899
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Biotechnology advances
- Accession number :
- edsair.doi.dedup.....085a39cc9605e225ed17cf8d014b341f