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Isolation and characterization of Listeria monocytogenes from the black-headed gull feces in Kunming, China
- Source :
- Journal of Infection and Public Health, Vol 11, Iss 1, Pp 59-63 (2018)
- Publication Year :
- 2016
-
Abstract
- Listeria monocytogenes is among the most important foodborne pathogens, which can be isolated from bird feces. The aim of this study was to examine the prevalence, genotypes, virulence factors and drug-resistance characteristics of L. monocytogenes isolated from the black-headed gull in Kunming, China. Serotypes and virulence genes were identified by polymerase chain reaction. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) methods were used to describe the genetic characteristics of all positive isolates. Furthermore, all L. monocytogenes isolates were tested for antimicrobial susceptibility by disk diffusion method. All isolates were positive for inlA, inlB, plcB, prfA, iap, actA and hly genes. Serotyping showed the isolates belonged to serotype 1/2b (2/9, 22%), 1/2c (3/9, 33%), and 4a (4/9, 44%). PFGE showed 4 different pulsotypes (PTs), and MLST differentiated the 9 isolates into 4 sequence types (STs), ST3 (1), ST5 (1), ST35 (3) and ST201 (4). The result of antimicrobial susceptibility revealed that all isolates were naturally sensitive to most antibiotics. The L. monocytogenes isolates had pathogenic potential. There is a potential infection risk for people who come to frequent contact with black-headed gulls in Kunming. Hence, it is necessary to conduct surveillance of L. monocytogenes in the migratory black-headed gulls, which would be valuable to prevent listeriosis in local residents and tourists. Keywords: Listeria monocytogenes, Virulence gene, PFGE, MLST, Antibiotic resistance
- Subjects :
- 0301 basic medicine
Serotype
China
Genotype
Virulence Factors
030106 microbiology
Virulence
Biology
medicine.disease_cause
Serogroup
Polymerase Chain Reaction
lcsh:Infectious and parasitic diseases
Microbiology
law.invention
03 medical and health sciences
Charadriiformes
Feces
Listeria monocytogenes
law
Disk Diffusion Antimicrobial Tests
Drug Resistance, Bacterial
medicine
Pulsed-field gel electrophoresis
Prevalence
Animals
lcsh:RC109-216
Listeriosis
Typing
Polymerase chain reaction
Bird Diseases
lcsh:Public aspects of medicine
Public Health, Environmental and Occupational Health
lcsh:RA1-1270
General Medicine
Virology
Electrophoresis, Gel, Pulsed-Field
Infectious Diseases
Multilocus sequence typing
Multilocus Sequence Typing
Subjects
Details
- ISSN :
- 1876035X
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of infection and public health
- Accession number :
- edsair.doi.dedup.....084890baef9bbd778d98420cc0e57cda