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Structural and functional characterization of Tomato SUMO1 gene
- Source :
- Saudi Journal of Biological Sciences, Saudi J Biol Sci, Saudi Journal of Biological Sciences, Vol 27, Iss 1, Pp 352-357 (2020)
- Publication Year :
- 2019
- Publisher :
- Elsevier, 2019.
-
Abstract
- Small ubiquitin-related modifier (SUMO) genes regulate various functions of target proteins through post-translational modification. The SUMO proteins have a similar 3-dimensional structure as that of ubiquitin proteins and occur through a cascade of enzymatic reactions. In the present study we have cloned a new SUMO gene from Tomato (Solanum lycopersicum L.), cv Saudi-1, named SlS-SUMO1 gene by PCR using specific primers. This gene has SUMO member's features such as C-terminal diglycine (GG) motif as processing site by ULP (ubiquitin-like SUMO protease) and has SUMO consensus ΨKXE/D sequence. Phylogenetic analysis showed that SlS-SUMO1 gene is highly conserved and homologous to Potatoes Ca-SUMO1 and Ca-SUMO2 genes based on sequence similarity. Expression protein of SlS-SUMO1 gene found to be localized in the nucleus, cytoplasm, and nuclear envelop or nuclear pore complex. SUMO conjugating enzyme SCE1a with SlS-SUMO1 protein co-expressed and co-localized in nucleus and formed nuclear subdomains. This study reported that the SlS-SUMO1 gene is a member of SUMO family and its SUMO protein processing using GG motif and activate and transport to nucleus through Sumoylation system in the plant cell. Keywords: SUMO genes, Solanum Lycopersicum L., Expression analysis, Bioinformatics
- Subjects :
- 0106 biological sciences
0301 basic medicine
Bioinformatics
medicine.medical_treatment
genetic processes
SUMO protein
macromolecular substances
01 natural sciences
environment and public health
Article
Expression analysis
03 medical and health sciences
Ubiquitin
medicine
Solanum Lycopersicum L
Nuclear pore
lcsh:QH301-705.5
Gene
chemistry.chemical_classification
Protease
biology
food and beverages
SUMO genes
Cell biology
enzymes and coenzymes (carbohydrates)
030104 developmental biology
Enzyme
medicine.anatomical_structure
lcsh:Biology (General)
chemistry
Cytoplasm
biology.protein
health occupations
Corrigendum
General Agricultural and Biological Sciences
Nucleus
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 22137106 and 1319562X
- Volume :
- 27
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Saudi Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....084697d39bf88b9f1a9387cdf000e9e1