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Iron affinity gel and gallium immobilized metal affinity chromatographic technique for phosphopeptide enrichment: a comparative study
- Source :
- Biotechnology & Biotechnological Equipment, Vol 31, Iss 3, Pp 639-646 (2017)
- Publication Year :
- 2017
-
Abstract
- Protein phosphorylation is central to most cellular processes. Despite the importance and widespread occurrence of this modification, phosphoproteome analysis of complex biological samples still remains a challenge. The present study has been undertaken to compare iron affinity immobilized metal ion affinity chromatography (Phos-Select™ IMAC) and Gallium immobilized metal ion affinity chromatography (Ga-IMAC) to enrich and characterize phosphopeptides from the whole-cell lysate of Jurkat cells. Our results indicated that enrichment was dependent on the isoelectric point (pI) and molecular mass of the proteins and it was found to be better in case of PHOS-Select IMAC (51.2%) as compared to Ga-IMAC (28.8%). This study compared and analysed phosphopeptide profile of Jurkat cells by two different IMAC techniques and provides advancement in phosphopeptide enrichment methods.
- Subjects :
- 0301 basic medicine
Lysis
Chromatography
Molecular mass
Phosphopeptide
lcsh:Biotechnology
chemistry.chemical_element
High-performance liquid chromatography
Jurkat cells
03 medical and health sciences
030104 developmental biology
Isoelectric point
Affinity chromatography
chemistry
iTRAQ
lcsh:TP248.13-248.65
Gallium
HPLC
LC-MS/MS
IMAC
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Biotechnology & Biotechnological Equipment, Vol 31, Iss 3, Pp 639-646 (2017)
- Accession number :
- edsair.doi.dedup.....08382c1f0bc00524b504b30c9d0d3ae9