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Infiltration of mesenchymal stem cells into PEGDA hydrogel
Infiltration of mesenchymal stem cells into PEGDA hydrogel
- Source :
- Bio-Medical Materials and Engineering. 24:1803-1815
- Publication Year :
- 2014
- Publisher :
- IOS Press, 2014.
-
Abstract
- BACKGROUND: Attachment of cells to fully hydrated hydrogel biomaterials, such as PEGDA, has proven challenging be-cause of the hydrophobic cellular membrane. OBJECTIVE: To demonstrate undifferentiated human mesenchymal stem cells (hMSCs) and hMSC-derived chondrocytesinfiltrated and attached to unmodified PEGDA hydrogel. METHODS: Human MSCs and MSC-Cys were cultured in and on PEGDA hydrogel. Attachment was verified by SEM andconfocal microscopy and was accompanied by vinculin expression, indicating the presence of focal contacts. Infiltration wasconfirmed by H&E and fluorescence staining. RESULTS: Cells cultured on top of PEGDA hydrogel infiltrated the material on the order of micrometers. CONCLUSIONS: These findings will aid in understanding the cell-scaffold interaction for regenerative medicine constructs.Keywords: Mesenchymal stem cells, PEGDA, vinculin, differentiation, chondrogenesis, infiltration 1. Introduction Articular cartilage is a metabolically active tissue that under normal conditions is maintained throughthe slow turnover of the extracellular matrix (ECM) by chondrocytes sparsely distributed throughout theECM [1]. Since cartilage tissue is avascular, once injured, it undergoes potentially irreversible necrosisrather than inflammation and repair as in vascularized tissues [2]. If a defect penetrates the underlyinglayer of subchondral bone, creating a full thickness defect or osteochondral lesion, limited repair occurs.However, this generally leads to the formation of less durable fibrocartilage rather than hyaline carti-lage [3]. When a cartilage defect is isolated, the ideal treatment strategy is to replicate normal tissueregeneration using autologous cells with the intention of creating a physiologically normal surface.
- Subjects :
- Materials science
Cell Survival
Biomedical Engineering
Biocompatible Materials
Regenerative medicine
Polyethylene Glycols
Biomaterials
Extracellular matrix
Cell Movement
Materials Testing
Cell Adhesion
medicine
Humans
Cells, Cultured
Hyaline
Focal Adhesions
Tissue Engineering
Tissue Scaffolds
biology
Cartilage
Mesenchymal stem cell
Hydrogels
Mesenchymal Stem Cells
General Medicine
Vinculin
medicine.disease
Chondrogenesis
Cell biology
medicine.anatomical_structure
biology.protein
Infiltration (medical)
Biomedical engineering
Subjects
Details
- ISSN :
- 09592989
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Bio-Medical Materials and Engineering
- Accession number :
- edsair.doi.dedup.....081f8ad1e0a8275af4ba49741720f3a6
- Full Text :
- https://doi.org/10.3233/bme-140991