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Infiltration of mesenchymal stem cells into PEGDA hydrogel

Infiltration of mesenchymal stem cells into PEGDA hydrogel

Authors :
Gregory Yourek
Jeremy J. Mao
Gwendolen C. Reilly
Xuejun Xin
Source :
Bio-Medical Materials and Engineering. 24:1803-1815
Publication Year :
2014
Publisher :
IOS Press, 2014.

Abstract

BACKGROUND: Attachment of cells to fully hydrated hydrogel biomaterials, such as PEGDA, has proven challenging be-cause of the hydrophobic cellular membrane. OBJECTIVE: To demonstrate undifferentiated human mesenchymal stem cells (hMSCs) and hMSC-derived chondrocytesinfiltrated and attached to unmodified PEGDA hydrogel. METHODS: Human MSCs and MSC-Cys were cultured in and on PEGDA hydrogel. Attachment was verified by SEM andconfocal microscopy and was accompanied by vinculin expression, indicating the presence of focal contacts. Infiltration wasconfirmed by H&E and fluorescence staining. RESULTS: Cells cultured on top of PEGDA hydrogel infiltrated the material on the order of micrometers. CONCLUSIONS: These findings will aid in understanding the cell-scaffold interaction for regenerative medicine constructs.Keywords: Mesenchymal stem cells, PEGDA, vinculin, differentiation, chondrogenesis, infiltration 1. Introduction Articular cartilage is a metabolically active tissue that under normal conditions is maintained throughthe slow turnover of the extracellular matrix (ECM) by chondrocytes sparsely distributed throughout theECM [1]. Since cartilage tissue is avascular, once injured, it undergoes potentially irreversible necrosisrather than inflammation and repair as in vascularized tissues [2]. If a defect penetrates the underlyinglayer of subchondral bone, creating a full thickness defect or osteochondral lesion, limited repair occurs.However, this generally leads to the formation of less durable fibrocartilage rather than hyaline carti-lage [3]. When a cartilage defect is isolated, the ideal treatment strategy is to replicate normal tissueregeneration using autologous cells with the intention of creating a physiologically normal surface.

Details

ISSN :
09592989
Volume :
24
Database :
OpenAIRE
Journal :
Bio-Medical Materials and Engineering
Accession number :
edsair.doi.dedup.....081f8ad1e0a8275af4ba49741720f3a6
Full Text :
https://doi.org/10.3233/bme-140991