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DNA extraction and amplification from Pinaceae dry wood
- Source :
- E-Prints Complutense: Archivo Institucional de la UCM, Universidad Complutense de Madrid, E-Prints Complutense. Archivo Institucional de la UCM, instname
- Publication Year :
- 2019
- Publisher :
- Sciendo, 2019.
-
Abstract
- Wood constitutes the unique source of DNA in dead trees, but extraction of adequate quality DNA from dry wood is usually challenging. However, many different molecular studies require the use of such DNA. We have standardized and validated a modified CTAB protocol to isolate DNA from dry wood from Abies pinsapo and Cedrus atlantica species. Due to the degradation and very little DNA that is normally present in the wood from dead trees we have developed a PCR based test to certify the quality of the extracted samples. In the present study, we have proved too the effectiveness of this methodology to isolate DNA from conifer dry wood samples of sufficient quality to perform further molecular genetic experiments.
- Subjects :
- 0106 biological sciences
0303 health sciences
biology
Cedrus atlantica
Extraction (chemistry)
technology, industry, and agriculture
Forestry
biology.organism_classification
01 natural sciences
DNA extraction
Genética
03 medical and health sciences
chemistry.chemical_compound
Horticulture
Plant science
chemistry
Pinaceae
Abies pinsapo
Genetics
Dead tree
DNA
030304 developmental biology
010606 plant biology & botany
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- E-Prints Complutense: Archivo Institucional de la UCM, Universidad Complutense de Madrid, E-Prints Complutense. Archivo Institucional de la UCM, instname
- Accession number :
- edsair.doi.dedup.....081d614b7d7c67ab69ba816892f546f9