Back to Search Start Over

FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway

Authors :
Linsen Zan
Xiaoyu Wang
Guohua Wang
Sayed Haidar Abbas Raza
Bingzhi Li
Chengcheng Liang
Wenzhen Zhang
Source :
Adipocyte, Vol 10, Iss 1, Pp 546-557 (2021), Adipocyte, article-version (VoR) Version of Record
Publication Year :
2021
Publisher :
Taylor & Francis Group, 2021.

Abstract

The family with sequence similarity 13 member A (FAM13A) gene has been discovered in recent years and is related to metabolism. In this study, the function of FAM13A in precursor adipocyte proliferation in Qinchuan cattle was investigated using fluorescence quantitative polymerase chain reaction (PCR), western blotting, 5-ethynyl-2′-deoxyuridine staining, and other tests. FAM13A promoted precursor adipocyte proliferation. To determine the pathway FAM13A was involved in, transcriptome sequencing, fluorescence quantitative PCR, western blotting, and other tests were used, which identified the hypoxia inducible factor-1 (HIF-1) signalling pathway. Finally, cobalt chloride, a chemical mimic of hypoxia, was used to treat precursor adipocytes. mRNA and protein levels of FAM13A were significantly increased after hypoxia. Thus, FAM13A promoted bovine precursor adipocyte proliferation by inhibiting the HIF-1 signalling pathway, whereas chemically induced hypoxia negatively regulated FAM13A expression, regulating cell proliferation.

Details

Language :
English
ISSN :
21623945
Volume :
10
Issue :
1
Database :
OpenAIRE
Journal :
Adipocyte
Accession number :
edsair.doi.dedup.....080af7c0162f37f1941d865b4c81d251