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FAM13A promotes proliferation of bovine preadipocytes by targeting Hypoxia-Inducible factor-1 signaling pathway
- Source :
- Adipocyte, Vol 10, Iss 1, Pp 546-557 (2021), Adipocyte, article-version (VoR) Version of Record
- Publication Year :
- 2021
- Publisher :
- Taylor & Francis Group, 2021.
-
Abstract
- The family with sequence similarity 13 member A (FAM13A) gene has been discovered in recent years and is related to metabolism. In this study, the function of FAM13A in precursor adipocyte proliferation in Qinchuan cattle was investigated using fluorescence quantitative polymerase chain reaction (PCR), western blotting, 5-ethynyl-2′-deoxyuridine staining, and other tests. FAM13A promoted precursor adipocyte proliferation. To determine the pathway FAM13A was involved in, transcriptome sequencing, fluorescence quantitative PCR, western blotting, and other tests were used, which identified the hypoxia inducible factor-1 (HIF-1) signalling pathway. Finally, cobalt chloride, a chemical mimic of hypoxia, was used to treat precursor adipocytes. mRNA and protein levels of FAM13A were significantly increased after hypoxia. Thus, FAM13A promoted bovine precursor adipocyte proliferation by inhibiting the HIF-1 signalling pathway, whereas chemically induced hypoxia negatively regulated FAM13A expression, regulating cell proliferation.
- Subjects :
- Hypoxia-Inducible Factor 1
Histology
Physiology
proliferation
Biology
Diseases of the endocrine glands. Clinical endocrinology
chemistry.chemical_compound
Adipocyte
Adipocytes
medicine
Animals
fam13a
QP1-981
Gene
Cell Proliferation
QH573-671
hypoxia
Cell Biology
Metabolism
Hypoxia (medical)
Hypoxia-Inducible Factor 1, alpha Subunit
RC648-665
Cell biology
chemistry
Cattle
hif-1 pathway
Signal transduction
medicine.symptom
Cytology
Function (biology)
Research Article
Research Paper
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 21623945
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Adipocyte
- Accession number :
- edsair.doi.dedup.....080af7c0162f37f1941d865b4c81d251