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in vitro generation of IFN-γ-producing Listeria-specific T cells is dependent on IFN-γ production by non-NK cells
- Source :
- Cellular Immunology. 160:211-216
- Publication Year :
- 1995
- Publisher :
- Elsevier BV, 1995.
-
Abstract
- In vitro 5-day cultures of naive spleen cells with viable Listeria monocytogenes (VLM), but not heat-killed L. monocytogenes, induced CD4+ T cells that produced IFN-gamma upon secondary antigen stimulation. The VLM-induced Listeria-specific T cells produced IFN-gamma but lacked expression of IL-2 and IL-4. To study the role of IFN-gamma in the induction of the IFN-gamma-producing T cells, we added anti-IFN-gamma mAb to the primary culture and analyzed IFN-gamma production upon secondary antigen stimulation. Addition of anti-IFN-gamma mAb to the culture suppressed generation of IFN-gamma-producing CD4+ T cells, suggesting that IFN-gamma is important in the induction of IFN-gamma-producing CD4+ T cells. Furthermore, our results showed that depletion of NK cells from spleen cells by anti-asialo GM1 antibody plus complement before culture enhanced induction of IFN-gamma-producing CD4+ T cells. Although NK cells are known to produce IFN-gamma, the results indicate that NK cell-derived IFN-gamma may not be important in induction of the Listeria-specific IFN-gamma-producing CD4+ T cells in the culture system. In addition, we demonstrated that IFN-gamma expression was high in CD4+ T cells from cultures of spleen cells with VLM at the primary culture level. These results suggest that IFN-gamma derived from T cells may enhance production of IFN-gamma by CD4+ T cells, while NK cells rather suppress the induction of IFN-gamma producing CD4+ T cells.
- Subjects :
- Hot Temperature
Sialoglycoproteins
Molecular Sequence Data
Immunology
Polymerase Chain Reaction
Interferon-gamma
Mice
Interleukin 21
T-Lymphocyte Subsets
Animals
Cytotoxic T cell
IL-2 receptor
Antigen-presenting cell
Cells, Cultured
Interleukin 3
Mice, Inbred BALB C
CD40
Base Sequence
biology
Antibodies, Monoclonal
T-Lymphocytes, Helper-Inducer
Natural killer T cell
Listeria monocytogenes
Cell biology
Killer Cells, Natural
Gene Expression Regulation
Culture Media, Conditioned
biology.protein
Interleukin 12
Interleukin-2
Osteopontin
Interleukin-4
Rabbits
Spleen
Subjects
Details
- ISSN :
- 00088749
- Volume :
- 160
- Database :
- OpenAIRE
- Journal :
- Cellular Immunology
- Accession number :
- edsair.doi.dedup.....080934d0be318fe955d61f8bbf955d91
- Full Text :
- https://doi.org/10.1016/0008-8749(95)80030-m