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Protective Role of Selenium in Immune-Relevant Cytokine and Immunoglobulin Production by Piglet Splenic Lymphocytes Exposed to Deoxynivalenol
- Source :
- Biological Trace Element Research. 184:83-91
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Deoxynivalenol (DON) is a mycotoxin that causes immunosuppression, especially in swine. Selenium (Se) is essential for proper functioning of the immune system in animals. However, little is known about the effects of DON and Se on cytokine or immunoglobulin production in piglets. Here, we addressed this gap by examining piglet splenic lymphocyte responses in vitro. Cells were stimulated with concanavalin A, a T cell stimulatory lectin, in the absence or presence of DON (0.1, 0.2, 0.4, and 0.8 μg/mL), Se (Na2SeO3, 2 μM), or combinations of Se 2 μM and DON 0.1–0.8 μg/mL for 12, 24, or 48 h. At each time point, supernatants and cells were collected and the expression of cytokine and immunoglobulin protein and mRNA was examined. Compared with control and Se-alone treatments, DON exposure significantly and dose dependently decreased the expression levels of IL-2, IL-4, IL-6, IL-10, IFN-γ, IgG, and IgM mRNA and protein. By contrast, co-treatment with DON + Se significantly increased the mRNA and protein levels of all factors examined, except IL-4 and IL-6, compared with DON treatment alone. The results of this investigation demonstrate that Se has the potential to counteract DON-induced immunosuppression in piglets and is a promising treatment for DON-mediated toxicity.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Swine
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
T cell
Clinical Biochemistry
Immunoglobulins
Biology
Biochemistry
Inorganic Chemistry
Selenium
03 medical and health sciences
Immune system
Internal medicine
medicine
Animals
Lymphocytes
Biochemistry (medical)
Immunosuppression
General Medicine
In vitro
030104 developmental biology
Endocrinology
Cytokine
medicine.anatomical_structure
Concanavalin A
Immunology
Toxicity
biology.protein
Cytokines
Antibody
Trichothecenes
Spleen
Subjects
Details
- ISSN :
- 15590720 and 01634984
- Volume :
- 184
- Database :
- OpenAIRE
- Journal :
- Biological Trace Element Research
- Accession number :
- edsair.doi.dedup.....0805693a569b0e0247c09a574a322c8a