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The role of somatostatin and its receptors (sstr2, sstr5) in the contractility of gilt inflamed uterus
- Source :
- Research in Veterinary Science. 133:163-173
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- We studied the inflammation effect on somatostatin receptors subtypes 2 (sstr2) and 5 (sstr5) expression in myometrium and somatostatin influence alone or with sstr2 and sstr5 antagonists on the contractility of gilt inflamed uterus. On day 3 of the estrous cycle, either E.coli suspension (E.coli group) or saline (SAL group) were injected into uterine horns. In the control pigs (CON group), only laparotomy was performed. Eight days later, in the E.coli group developed severe acute endometritis. In this group, myometrial sstr2 mRNA expression lowered and protein expression increased compared to other groups. Compared to period before somatostatin administration, somatostatin did not change tension in myometrium and endometrium/myometrium of three groups, reduced amplitude and frequency in the CON and SAL groups, and increased amplitude and decreased frequency in the E.coli group. In this group, amplitude was increased by somatostatin compared to other groups. In the CON and SAL groups, sstr2 eliminated inhibitory somatostatin effect on amplitude, while sstr5 antagonist reversed inhibitory somatostatin effect on amplitude. In the E.coli group, sstr2 antagonist reversed stimulatory somatostatin effect on amplitude, while in sstr5 antagonist presence stimulatory somatostatin effect was more deepened compared to somatostatin action alone. After using sstr2 antagonist more deepened inhibitory somatostatin effect on frequency in the CON and E.coli groups was found. Sstr5 antagonist partly eliminated inhibitory somatostatin effect on frequency in the SAL group. Summarizing, the uterine inflammation increases the myometrial sstr2 protein expression; somatostatin raises amplitude of the inflamed uterus acting by sstr2, while drops this parameter by sstr5.
- Subjects :
- endocrine system
medicine.medical_specialty
Swine
040301 veterinary sciences
Sus scrofa
Anti-Inflammatory Agents
Uterus
Endometrium
0403 veterinary science
Uterine Contraction
03 medical and health sciences
Internal medicine
medicine
Animals
Somatostatin receptor 2
Receptors, Somatostatin
Escherichia coli Infections
030304 developmental biology
Inflammation
Swine Diseases
Uterine Diseases
0303 health sciences
General Veterinary
Chemistry
Somatostatin receptor
Myometrium
Antagonist
Uterine horns
04 agricultural and veterinary sciences
Hormones
medicine.anatomical_structure
Endocrinology
Somatostatin
Gene Expression Regulation
Female
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 00345288
- Volume :
- 133
- Database :
- OpenAIRE
- Journal :
- Research in Veterinary Science
- Accession number :
- edsair.doi.dedup.....07febc0d79632f9304eb58cea479e4fa