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Expression of CAS/CSE1L, the cellular apoptosis susceptibility protein, correlates with neoplastic progression in Barrett’s esophagus
- Publication Year :
- 2018
-
Abstract
- BACKGROUND: Identifying the molecular switch responsible for the neoplastic progression of Barrett’s esophagus (BE) and initiation of adenocarcinoma is clinically essential and it will have a profound impact on patient diagnosis, prognosis, and treatment. The cellular apoptosis susceptibility gene CAS/CSE1L is overexpressed in various cancers, including a rare report on esophageal adenocarcinoma; however, its expression in BE neoplasia has not been addressed. METHODS: We investigated the expression of the CAS/CSE1L protein immunohistochemically in 56 esophageal resection specimens for adenocarcinoma arising in BE. For each specimen, a full representative section of the invasive adenocarcinoma (ADC) was selected to include, when possible, BE, low grade dysplasia (LGD) and high grade dysplasia (HGD). Samples were stained for CAS/CSE1L expression using a rabbit polyclonal antibody recognizing the N-terminus of human CAS/CSE1L. Protein expression levels were measured using the Allred semiquantitative scoring system. The data was evaluated using chi-squared statistical analysis. Gene expression Omnibus was queried for CAS/CSE1L and BE neoplasia. RESULTS: We found minimal to absent CAS/CSE1L in all BE tissue samples; however, CAS/CSE1L was upregulated in 60% of LGD and overexpressed in HGD and ADC. The results were statistically significant (P< 0.05). The localization of CAS/CSE1L protein was nuclear in BE; it became nuclear and cytoplasmic in LGD and HGD, and predominantly cytoplasmic in ADC. A similar progressive increase was observed for CAS/CSE1L gene expression. CONCLUSION: These findings show changes in CAS/CSE1L during BE progression. CAS/CSE1L may represent a potential marker for dysplasia/carcinoma.
- Subjects :
- 0301 basic medicine
Adult
Male
Pathology
medicine.medical_specialty
Histology
Esophageal Neoplasms
Adenocarcinoma
Article
Pathology and Forensic Medicine
03 medical and health sciences
Barrett Esophagus
0302 clinical medicine
Esophagus
Downregulation and upregulation
Cellular Apoptosis Susceptibility Protein
medicine
Carcinoma
Biomarkers, Tumor
Humans
Gene
Aged
Aged, 80 and over
Hyperplasia
business.industry
Middle Aged
medicine.disease
humanities
Neoplasm Proteins
Gene Expression Regulation, Neoplastic
Medical Laboratory Technology
030104 developmental biology
medicine.anatomical_structure
Dysplasia
030220 oncology & carcinogenesis
Barrett's esophagus
Female
business
Cellular apoptosis susceptibility protein
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....07eaeef30ffc16312784ded867c5cedf