MOESM3 of Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation

Additional file 3: Figure S2. H1 variant expression and phosphorylation in differentiating NT2 cells. Crude H1 in acid extracts of nuclei isolated from NT2 cells induced to differentiate with 10 μM retinoic acid for 0, 1, 3 or 7 days was fractionated by hydrophobic interaction chromatography (HIC). Eluate absorbance at 214 nm (Y axis) is plotted relative to time (X axis) for equivalent portions of each separation. The relative elution positions of H1.2, H1.3, H1.4 and H1.5 and the phosphorylation stoichiometry of their major interphase forms, as characterized previously [23], are indicated above the 0 day trace. H1.0 coelutes as a broad peak that overlaps with both phosphorylated and non-phosphorylated H1.5 (data not shown).