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Integrating gold nanoclusters, folic acid and reduced graphene oxide for nanosensing of glutathione based on 'turn-off' fluorescence
Integrating gold nanoclusters, folic acid and reduced graphene oxide for nanosensing of glutathione based on 'turn-off' fluorescence
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, Scientific Reports, Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021), Dipòsit Digital de Documents de la UAB, Universitat Autònoma de Barcelona
- Publication Year :
- 2021
-
Abstract
- Glutathione (GSH) is a useful biomarker in the development, diagnosis and treatment of cancer. However, most of the reported GSH biosensors are expensive, time-consuming and often require complex sample treatment, which limit its biological applications. Herein, a nanobiosensor for the detection of GSH using folic acid-functionalized reduced graphene oxide-modified BSA gold nanoclusters (FA-rGO-BSA/AuNCs) based on the fluorescence quenching interactions is presented. Firstly, a facile and optimized protocol for the fabrication of BSA/AuNCs is developed. Functionalization of rGO with folic acid is performed using EDC/NHS cross-linking reagents, and their interaction after loading with BSA/AuNCs is demonstrated. The formation of FA-rGO, BSA/AuNCs and FA-rGO-BSA/AuNCs are confirmed by the state-of-art characterization techniques. Finally, a fluorescence turn-off sensing strategy is developed using the as-synthesized FA-rGO-BSA/AuNCs for the detection of GSH. The nanobiosensor revealed an excellent sensing performance for the detection of GSH with high sensitivity and desirable selectivity over other potential interfering species. The fluorescence quenching is linearly proportional to the concentration of GSH between 0 and 1.75 µM, with a limit of detection of 0.1 µM under the physiological pH conditions (pH 7.4). Such a sensitive nanobiosensor paves the way to fabricate a “turn-on” or “turn-off” fluorescent sensor for important biomarkers in cancer cells, presenting potential nanotheranostic applications in biological detection and clinical diagnosis.<br />This work was supported by the Fundamental Research Grant Scheme (FRGS), Ministry of Higher Education (MOHE), Malaysia [FRGS/1/2016/STG07/UNIM/02/1] and by the CERCA Programme/Generalitat de Catalunya. ICN2 is supported by the Severo Ochoa Centres of Excellence programme, funded by the Spanish Research Agency (AEI, Grant no. SEV-2017-0706). We acknowledge support from Ministerio de Economía, Industria y Competitividad (MINECO), Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) for funding the project MAT2017-87202-P. Authors acknowledge the European Union’s Horizon 2020 research and innovation programme under Grant Agreement No. 881603 (Graphene Flagship Core 3).
- Subjects :
- Science
02 engineering and technology
010402 general chemistry
01 natural sciences
Article
Nanoclusters
law.invention
Biomaterials
chemistry.chemical_compound
law
Nanoscience and technology
Detection limit
Multidisciplinary
Graphene
Chemistry
Nanobiotechnology
Glutathione
021001 nanoscience & nanotechnology
Combinatorial chemistry
Fluorescence
0104 chemical sciences
3. Good health
Reagent
Surface modification
Medicine
0210 nano-technology
Biosensor
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, Scientific Reports, Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021), Dipòsit Digital de Documents de la UAB, Universitat Autònoma de Barcelona
- Accession number :
- edsair.doi.dedup.....07c7492b2998773191155a4cc37c3c7e