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Modulating DNA Translocation by a Controlled Deformation of a PDMS Nanochannel Device
- Source :
- Scientific reports (Nature Publishing Group) 2 (2012): 791. doi:10.1038/srep00791, info:cnr-pdr/source/autori:Paola Fanzio1, Chiara Manneschi1*, Elena Angeli1, Valentina Mussi1, Giuseppe Firpo1, Luca Ceseracciu2, Luca Repetto1 & Ugo Valbusa1/titolo:Modulating DNA translocation by a controlled deformation of a PDMS nanochannel device/doi:10.1038%2Fsrep00791/rivista:Scientific reports (Nature Publishing Group)/anno:2012/pagina_da:791/pagina_a:/intervallo_pagine:791/volume:2, Scientific Reports
- Publication Year :
- 2012
-
Abstract
- Several strategies have been developed for the control of DNA translocation in nanopores and nanochannels. However, the possibility to reduce the molecule speed is still challenging for applications in the field of single molecule analysis, such as ultra-rapid sequencing. This paper demonstrates the possibility to alter the DNA translocation process through an elastomeric nanochannel device by dynamically changing its cross section. More in detail, nanochannel deformation is induced by a macroscopic mechanical compression of the polymeric device. This nanochannel squeezing allows slowing down the DNA molecule passage inside it. This simple and low cost method is based on the exploitation of the elastomeric nature of the device, can be coupled with different sensing techniques, is applicable in many research fields, such as DNA detection and manipulation, and is promising for further development in sequencing technology.
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Scientific reports (Nature Publishing Group) 2 (2012): 791. doi:10.1038/srep00791, info:cnr-pdr/source/autori:Paola Fanzio1, Chiara Manneschi1*, Elena Angeli1, Valentina Mussi1, Giuseppe Firpo1, Luca Ceseracciu2, Luca Repetto1 & Ugo Valbusa1/titolo:Modulating DNA translocation by a controlled deformation of a PDMS nanochannel device/doi:10.1038%2Fsrep00791/rivista:Scientific reports (Nature Publishing Group)/anno:2012/pagina_da:791/pagina_a:/intervallo_pagine:791/volume:2, Scientific Reports
- Accession number :
- edsair.doi.dedup.....07c6561212affb0981d5ff063e40e198