Supplementary Figure 7 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression

PDF file - 67K, Control or PIP4K2B knockdown MCF10A cells were labelled with 32P-orthophosphate for two hours after which lipids were extracted, deacetylated and the lipid head groups were separated by HPLC. Radioactivity was quantified using an online detector and the data are presented as counts in each head group divided by the total counts + the SD. B. Lipids were extracted from control or PIP4K2B knockdown MCF10A cells and the abundance of PtdIns5P was measured. The data are presented as the pmoles of PtdIns5P/nmol of total lipid phosphate +the SD.