Back to Search
Start Over
In vitro effects of metals on isolated head-kidney and blood leucocytes of the teleost fish Sparus aurata L. and Dicentrarchus labrax L
- Source :
- Fish & Shellfish Immunology. 54:77-85
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The in vitro use of fish leucocytes to test the toxicity of aquatic pollutants, and particularly the immutoxicological effects, could be a valuable alternative to fish bioassays but has received little attention. In this study, head-kidney and peripheral blood leucocytes (HKLs and PBLs, respectively) from gilthead seabream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) specimens were exposed to Cd, MeHg (methylmercury), Pb or As for 24 h being evaluated the resulting cytotoxicity. Exposure to metals produced a dose-dependent reduction in the viability, and MeHg showed the highest toxicity followed by Cd, As and Pb. Interestingly, leucocytes from European sea bass are more resistant to metal exposure than those from gilthead seabream. Similarly, HKLs are always more sensitive than those isolated from blood from the same fish species. Moreover, fish leucocytes incubated with metals exhibited alterations in gene expression profiles that were more pronounced in the HKLs in general, being Pb the metal provoking less effects. Concretely, genes related to cellular protection (metallothionein), stress (heat shock protein 70) and oxidative stress (superoxide dismutase, catalase and glutathione reductase) were, in general, down-regulated in seabream HKLs but up-regulated in seabream PBLs and sea bass HKLs and PBLs. In addition, this profile leads to the increase of expression in genes related to apoptosis (Bcl2 associated X protein and caspase 3). Finally, transcription of genes involved in immunity (interleukin-1β and immunoglobulin M) was down-regulated, mainly in seabream leucocytes. This study points to the benefits for evaluating the toxicological mechanisms of marine pollution using fish leucocytes in vitro and insight into the mechanisms at gene level.
- Subjects :
- Fish Proteins
0301 basic medicine
Glutathione reductase
Apoptosis
010501 environmental sciences
Aquatic Science
01 natural sciences
Arsenic
Superoxide dismutase
03 medical and health sciences
Metals, Heavy
Leukocytes
Animals
Environmental Chemistry
Metallothionein
Sea bass
0105 earth and related environmental sciences
biology
General Medicine
Head Kidney
biology.organism_classification
Molecular biology
Immunity, Innate
Sea Bream
Oxidative Stress
030104 developmental biology
Gene Expression Regulation
Cytoprotection
Catalase
Toxicity
Immunology
biology.protein
Bass
Dicentrarchus
Water Pollutants, Chemical
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....07819968f7a71f3bcde72abd9a076c9a