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Hyperleptinemia depletes fat from denervated fat tissue
- Source :
- Biochemical and biophysical research communications. 260(3)
- Publication Year :
- 1999
-
Abstract
- Adenovirus-mediated transfer of the leptin gene causes severe hyperleptinemia with rapid disappearance of visible body fat. To determine if this dramatic lipopenic action is mediated by neurotransmitted signals from the central nervous system, we transplanted the right epididymal fat pad of normal rats to the anterior abdominal wall. Four weeks later, rats were infused with either adenovirus-leptin cDNA (AdCMV-leptin) or adenovirus-beta-galactosidase (AdCMV-beta-gal). Eight days later, plasma leptin averaged 23 +/- 12 ng/ml in the former and 1.2 +/- 0.4 ng/ml in the latter. The fat transplant was intact in all 4 AdCMV-beta-gal-infused rats but had disappeared in all 4 hyperleptinemic rats. Tyrosine hydroxylase staining of the fat pad remnant was negative, excluding regrowth of sympathetic nerves. Thus, the lipopenic action of severe hyperleptinemia on adipocytes is not mediated by neurotransmitters, but must have resulted either from direct action of leptin and/or from leptin-mediated neurohormones.
- Subjects :
- Leptin
medicine.medical_specialty
Time Factors
Tyrosine 3-Monooxygenase
Central nervous system
Biophysics
Adipose tissue
Biology
Biochemistry
Transplantation, Autologous
Fat pad
Adenoviridae
Abdominal wall
Rats, Sprague-Dawley
Internal medicine
medicine
Animals
Rats, Wistar
Sympathectomy
Molecular Biology
Tyrosine hydroxylase
digestive, oral, and skin physiology
Gene Transfer Techniques
Proteins
Cell Biology
Staining
Nerve Regeneration
Rats
Endocrinology
medicine.anatomical_structure
Adipose Tissue
Ventromedial Hypothalamic Nucleus
Neurohormones
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 260
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....07794f5d7dd825aeab44dd6f1942655c