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Validation of a geropathology grading system for aging mouse studies
- Source :
- GeroScience. 41:455-465
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- An understanding of early-onset mechanisms underlying age-related changes can be obtained by evaluating changes that precede frailty and end of life using histological characterization of age-related lesions. Histopathology-based information as a component of aging studies in mice can complement and add context to molecular, cellular, and physiologic data, but there is a lack of information regarding scoring criteria and lesion grading guidelines. This report describes the validation of a grading system, designated as the geropathology grading platform (GGP), which generated a composite lesion score (CLS) for comparison of histological lesion scores in tissues from aging mice. To assess reproducibility of the scoring system, multiple veterinary pathologists independently scored the same slides from the heart, lung, liver, and kidney from two different strains (C57BL/6 and CB6F1) of male mice at 8, 16, 24, and 32 months of age. There was moderate to high agreement between pathologists, particularly when agreement within a 1-point range was considered. CLS for all organs was significantly higher in older versus younger mice, suggesting that the GGP was reliable for detecting age-related pathology in mice. The overall results suggest that the GGP guidelines reliably distinguish between younger and older mice and may therefore be accurate in distinguishing between experimental groups of mice with more, or less, age-related pathology.
- Subjects :
- Aging
Pathology
medicine.medical_specialty
Lung
Geroscience
business.industry
Myocardium
Scoring criteria
Male mice
Kidney
Molecular medicine
Mice, Inbred C57BL
Lesion
medicine.anatomical_structure
Liver
Models, Animal
Animals
Medicine
Original Article
Histopathology
Geriatrics and Gerontology
medicine.symptom
business
Grading (education)
Subjects
Details
- ISSN :
- 25092723 and 25092715
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- GeroScience
- Accession number :
- edsair.doi.dedup.....0776a1ead66cf73d0cf5507224304c2c