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Exploration of exosomal microRNA expression profiles in pigeon ‘Milk’ during the lactation period
- Source :
- BMC Genomics, Vol 19, Iss 1, Pp 1-12 (2018), BMC Genomics
- Publication Year :
- 2018
- Publisher :
- BMC, 2018.
-
Abstract
- Background Pigeon crop has the unique ability to produce a nutrient rich substance termed pigeon ‘milk’ (PM), which has functional resemblance with the mammalian milk. Previous researches have demonstrated that a large number of exosomes and exosomal miRNAs exist in mammalian milk, and many of them are associated with immunity, growth and development. However, to date, little is known about the exosomes and exosomal miRNAs in PM. Results In this study, we isolated the exosomes from PM and used small RNA sequencing to investigate the distribution and expression profiles of exosomal miRNAs. A total of 301 mature miRNAs including 248 conserved and 53 novel miRNAs were identified in five lactation stages i.e. 1d, 5d, 10d, 15d, and 20d. From these, four top 10 conserved miRNAs (cli-miR-21-5p, cli-miR-148a-3p, cli-miR-10a-5p and cli-miR-26a-5p) were co-expressed in all five stages. We speculate that these miRNAs may have important role in the biosynthesis and metabolism of PM. Moreover, similar to the mammalian milk, a significant proportion of immune and growth-related miRNAs were also present and enriched in PM exosomes. Furthermore, we also identified 41 orthologous miRNAs group (giving rise to 81 mature miRNA) commonly shared with PM, human, bovine and porcine breast milk. Additionally, functional enrichment analysis revealed the role of exosomal miRNAs in organ development and in growth-related pathways including the MAPK, Wnt and insulin pathways. Conclusions To sum-up, this comprehensive analysis will contribute to a better understanding of the underlying functions and regulatory mechanisms of PM in squabs. Electronic supplementary material The online version of this article (10.1186/s12864-018-5201-0) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Bodily Secretions
Small RNA
Time Factors
lcsh:QH426-470
Swine
lcsh:Biotechnology
Biology
Proteomics
Exosomes
03 medical and health sciences
Species Specificity
Lactation
lcsh:TP248.13-248.65
microRNA
Genetics
medicine
Animals
Humans
Columbidae
Gene
Pigeon ‘milk’
Milk, Human
Gene Expression Profiling
Wnt signaling pathway
Microvesicles
Cell biology
MicroRNAs
lcsh:Genetics
Gene Ontology
Milk
030104 developmental biology
medicine.anatomical_structure
miRNAs
Cattle
Female
DNA microarray
Small RNA-seq
Research Article
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 14712164
- Volume :
- 19
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Genomics
- Accession number :
- edsair.doi.dedup.....0763c9a95ef58bbed34604c02c03e920