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Characterization of fructose-1,6-bisphosphate aldolase during anoxia in the tolerant turtle, Trachemys scripta elegans: an assessment of enzyme activity, expression and structure
Characterization of fructose-1,6-bisphosphate aldolase during anoxia in the tolerant turtle, Trachemys scripta elegans: an assessment of enzyme activity, expression and structure
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 7, p e68830 (2013)
- Publication Year :
- 2013
- Publisher :
- Public Library of Science, 2013.
-
Abstract
- One of the most adaptive facultative anaerobes among vertebrates is the freshwater turtle, Trachemys scripta elegans. Upon a decrease in oxygen supply and oxidative phosphorylation, these turtles are able to reduce their metabolic rate and recruit anaerobic glycolysis to meet newly established ATP demands. Within the glycolytic pathway, aldolase enzymes cleave fructose-1,6-bisphosphate to triose phosphates facilitating an increase in anaerobic production of ATP. Importantly, this enzyme exists primarily as tissue-specific homotetramers of aldolase A, B or C located in skeletal muscle, liver and brain tissue, respectively. The present study characterizes aldolase activity and structure in the liver tissue of a turtle whose survival greatly depends on increased glycolytic output during anoxia. Immunoblot and mass spectrometry analysis verified the presence of both aldolase A and B in turtle liver tissue, and results from co-immunoprecipitation experiments suggested that in the turtle aldolase proteins may exist as an uncommon heterotetramer. Expression levels of aldolase A protein increased significantly in liver tissue to 1.59±0.11-fold after 20 h anoxia, when compared to normoxic control values (P
- Subjects :
- Models, Molecular
Fructose 1,6-bisphosphate
Anatomy and Physiology
Protein Conformation
Enzyme Metabolism
Fructose-bisphosphate aldolase
lcsh:Medicine
Biochemistry
chemistry.chemical_compound
0302 clinical medicine
Tandem Mass Spectrometry
Fructose-Bisphosphate Aldolase
Molecular Cell Biology
Glycolysis
Anaerobiosis
lcsh:Science
Hypoxia
0303 health sciences
Multidisciplinary
biology
Enzyme Classes
Enzymes
Turtles
Liver
030220 oncology & carcinogenesis
Medicine
Female
Metabolic Pathways
Rabbits
Research Article
Immunoblotting
Molecular Sequence Data
Oxidative phosphorylation
Gene Expression Regulation, Enzymologic
Enzyme Regulation
03 medical and health sciences
Metabolic Networks
Animals
Immunoprecipitation
Amino Acid Sequence
Biology
030304 developmental biology
Enzyme Kinetics
Aldolase B
Aldolase A
lcsh:R
Computational Biology
Heterotetramer
Kinetics
Metabolism
chemistry
Anaerobic glycolysis
biology.protein
lcsh:Q
Physiological Processes
Energy Metabolism
Sequence Alignment
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Vol 8, Iss 7, p e68830 (2013)
- Accession number :
- edsair.doi.dedup.....075f378a9f81b4f8c724fb4d101c7038