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Low levels of faecal lactobacilli in women with iron-deficiency anaemia in south India
- Source :
- The British journal of nutrition. 104(7)
- Publication Year :
- 2010
-
Abstract
- Fe deficiency in women contributes significantly to maternal and child morbidity in India. The intestinal bacterial flora may facilitate absorption of Fe from the caecum and proximal colon. The present study investigated the possibility that intestinal microbiota of anaemic young women may differ from that of women with normal Hb levels. The microbiota was quantified by real-time PCR in faeces of eight anaemic (Hb ≤ 100 g/l) and twenty-six normohaemic (Hb ≥ 120 g/l) women aged 18–25 years. Sequences of 16S ribosomal DNA (rDNA) specific to Bifidobacterium genus, Lactobacillus acidophilus group, Bacteroides–Prevotella–Porphyromonas group, Clostridium leptum group and Eubacterium rectale were amplified and expressed (as relative difference) relative to the universally conserved bacterial 16S rDNA sequences. Dietary intakes of energy, carbohydrate, fibre and Fe were ascertained by maintenance of a diet diary for a week. Faecal lactobacilli were significantly lower in anaemic women (median 6·6 × 10− 8, relative difference compared with total bacteria) than in the reference group (2·9 × 10− 6; P = 0·001, unpaired t test with logarithmic transformation). There was no difference between the two groups with respect to any of the other bacteria that were examined. Intakes of energy, carbohydrate, fibre, Fe and milk were similar in both the groups. Fe deficiency in young women in south India was associated with low levels of lactobacilli in the faeces. The relationship between lactobacilli and Fe deficiency needs to be explored further.
- Subjects :
- Adult
DNA, Bacterial
Adolescent
Colon
Colony Count, Microbial
Medicine (miscellaneous)
Physiology
India
Microbiology
Caecum
Feces
Hemoglobins
Young Adult
Lactobacillus acidophilus
Reference Values
Lactobacillus
RNA, Ribosomal, 16S
medicine
Humans
Bifidobacterium
Nutrition and Dietetics
biology
Anemia, Iron-Deficiency
Bacteria
Reverse Transcriptase Polymerase Chain Reaction
Clostridium leptum
Iron deficiency
biology.organism_classification
medicine.disease
Diet Records
Diet
Iron-deficiency anemia
Female
Subjects
Details
- ISSN :
- 14752662
- Volume :
- 104
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- The British journal of nutrition
- Accession number :
- edsair.doi.dedup.....07494efaf622c73c2fa4bc5c1c293559