Back to Search Start Over

Low levels of faecal lactobacilli in women with iron-deficiency anaemia in south India

Authors :
Ramadass Balamurugan
R. Shobana Devi
R. Regina Mary
Hepsiba Jancy
Balakrishnan S. Ramakrishna
Sucharita Chittaranjan
Source :
The British journal of nutrition. 104(7)
Publication Year :
2010

Abstract

Fe deficiency in women contributes significantly to maternal and child morbidity in India. The intestinal bacterial flora may facilitate absorption of Fe from the caecum and proximal colon. The present study investigated the possibility that intestinal microbiota of anaemic young women may differ from that of women with normal Hb levels. The microbiota was quantified by real-time PCR in faeces of eight anaemic (Hb ≤ 100 g/l) and twenty-six normohaemic (Hb ≥ 120 g/l) women aged 18–25 years. Sequences of 16S ribosomal DNA (rDNA) specific to Bifidobacterium genus, Lactobacillus acidophilus group, Bacteroides–Prevotella–Porphyromonas group, Clostridium leptum group and Eubacterium rectale were amplified and expressed (as relative difference) relative to the universally conserved bacterial 16S rDNA sequences. Dietary intakes of energy, carbohydrate, fibre and Fe were ascertained by maintenance of a diet diary for a week. Faecal lactobacilli were significantly lower in anaemic women (median 6·6 × 10− 8, relative difference compared with total bacteria) than in the reference group (2·9 × 10− 6; P = 0·001, unpaired t test with logarithmic transformation). There was no difference between the two groups with respect to any of the other bacteria that were examined. Intakes of energy, carbohydrate, fibre, Fe and milk were similar in both the groups. Fe deficiency in young women in south India was associated with low levels of lactobacilli in the faeces. The relationship between lactobacilli and Fe deficiency needs to be explored further.

Details

ISSN :
14752662
Volume :
104
Issue :
7
Database :
OpenAIRE
Journal :
The British journal of nutrition
Accession number :
edsair.doi.dedup.....07494efaf622c73c2fa4bc5c1c293559