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Combined microRNA and mRNA detection in mammalian retinas by in situ hybridization chain reaction
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- Improved in situ hybridization methods for mRNA detection in tissues have been developed based on the hybridization chain reaction (HCR). We show that in situ HCR methods can be used for the detection of microRNAs in tissue sections from mouse retinas. In situ HCR can be used for the detection of two microRNAs simultaneously or for the combined detection of microRNA and mRNA. In addition, miRNA in situ HCR can be combined with immunodetection of proteins. We use these methods to characterize cells expressing specific microRNAs in the mouse retina. We find that miR-181a is expressed in amacrine cells during development and in adult retinas, and it is present in both GABAergic and glycinergic amacrine cells. The detection of microRNAs with in situ HCR should facilitate studies of microRNA function and gene regulation in the retina and other tissues.
- Subjects :
- 0301 basic medicine
In situ
lcsh:Medicine
In situ hybridization
Article
Retina
Mice
03 medical and health sciences
0302 clinical medicine
microRNA
medicine
Animals
RNA, Messenger
lcsh:Science
In Situ Hybridization
Regulation of gene expression
Messenger RNA
Multidisciplinary
Chemistry
lcsh:R
Cell type diversity
RNA
3. Good health
Cell biology
MicroRNAs
Amacrine Cells
030104 developmental biology
medicine.anatomical_structure
miRNAs
lcsh:Q
human activities
030217 neurology & neurosurgery
Function (biology)
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....07465cf2b47acd139fc5172fac23b5c1