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Application of dried blood spots to determine vitamin D status in a large nutritional study with unsupervised sampling: the Food4Me project
- Source :
- British Journal of Nutrition, 115(2), 202-211. Cambridge University Press
- Publication Year :
- 2016
-
Abstract
- An efficient and robust method to measure vitamin D (25-hydroxy vitamin D3(25(OH)D3) and 25-hydroxy vitamin D2in dried blood spots (DBS) has been developed and applied in the pan-European multi-centre, internet-based, personalised nutrition intervention study Food4Me. The method includes calibration with blood containing endogenous 25(OH)D3, spotted as DBS and corrected for haematocrit content. The methodology was validated following international standards. The performance characteristics did not reach those of the current gold standard liquid chromatography-MS/MS in plasma for all parameters, but were found to be very suitable for status-level determination under field conditions. DBS sample quality was very high, and 3778 measurements of 25(OH)D3were obtained from 1465 participants. The study centre and the season within the study centre were very good predictors of 25(OH)D3levels (P3level on 20 January and a maximum on 21 July. The seasonal amplitude varied from centre to centre. The largest difference between winter and summer levels was found in Germany and the smallest in Poland. The model was cross-validated to determine the consistency of the predictions and the performance of the DBS method. The Pearson’s correlation between the measured values and the predicted values wasr0·65, and thesdof their differences was 21·2 nmol/l. This includes the analytical variation and the biological variation within subjects. Overall, DBS obtained by unsupervised sampling of the participants at home was a viable methodology for obtaining vitamin D status information in a large nutritional study.
- Subjects :
- Male
Medicine (miscellaneous)
Vitamin D status
Hematocrit
RETINOL
01 natural sciences
SERUM
chemistry.chemical_compound
0302 clinical medicine
Reference Values
ASSAY
25-Hydroxyvitamin D 2
Nutrition and Dietetics
medicine.diagnostic_test
Sampling (statistics)
Middle Aged
Calibration
Female
TURNOVER
Seasons
HEMATOCRIT
Vitamin
Adult
Paper
medicine.medical_specialty
Adolescent
Diet therapy
Nutritional Status
030209 endocrinology & metabolism
Sensitivity and Specificity
vitamin D deficiency
03 medical and health sciences
Animal science
Analytical methods
medicine
Vitamin D and neurology
Humans
Desiccation
Aged
Calcifediol
business.industry
Remote Consultation
010401 analytical chemistry
Reproducibility of Results
Gold standard (test)
medicine.disease
Vitamin D Deficiency
0104 chemical sciences
Surgery
Nutrition Assessment
chemistry
Nutrition intervention study Food4Me
Reagent Kits, Diagnostic
Dried blood spot sampling
business
Diet Therapy
Subjects
Details
- Language :
- English
- ISSN :
- 00071145
- Volume :
- 115
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- British Journal of Nutrition
- Accession number :
- edsair.doi.dedup.....0742b3b3d023a31b1d6f77317737620c