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Distribution of γ-hexachlorocyclohexane-degrading genes on three replicons inSphingobium japonicumUT26
- Source :
- FEMS Microbiology Letters. 256:112-118
- Publication Year :
- 2006
- Publisher :
- Oxford University Press (OUP), 2006.
-
Abstract
- Sphingobium japonicum (formerly Sphingomonas paucimobilis) UT26 utilizes the important insecticide gamma-hexachlorocyclohexane as a sole source of carbon and energy. In previous studies, we isolated and characterized six structural genes (linA to linF) and one regulatory gene (linR) of UT26 for the degradation of gamma-hexachlorocyclohexane to beta-ketoadipate. Our analysis in this study indicated that the UT26 genome consists of three large circular replicons of 3.6 Mb, 670 kb, and 185 kb. The 3.6 Mb and the 670 kb replicons had one and two copies, respectively, of the 16S ribosomal RNA gene, and these replicons were designated as chromosomes (Chr) I and II, respectively. Chr I was indicated to be a main chromosome carrying the dnaA gene. The first three lin genes, linA to linC, for conversion of gamma-hexachlorocyclohexane to 2,5-dichlorohydroquinone, were dispersed on Chr I. The 185 kb plasmid, pCHQ1, carried the linRED operon for the conversion of 2,5-dichlorohydroquinone to maleylacetate and was conjugatively transferred to another sphingomonad strain. The linF gene encoding maleylacetate reductase was located on Chr II. These results indicated that the genes for the complete gamma-hexachlorocyclohexane degradation are dispersed on the three large replicons of UT26.
- Subjects :
- Sphingomonas paucimobilis
Maleylacetate reductase
Operon
Molecular Sequence Data
Microbiology
Plasmid
Bacterial Proteins
RNA, Ribosomal, 16S
Gene Order
Genetics
Molecular Biology
Gene
Regulator gene
Base Sequence
biology
Structural gene
Chromosome Mapping
DNA Restriction Enzymes
biology.organism_classification
Molecular biology
Electrophoresis, Gel, Pulsed-Field
DNA-Binding Proteins
Sphingomonadaceae
Conjugation, Genetic
Sphingobium japonicum
Replicon
Genome, Bacterial
Hexachlorocyclohexane
Subjects
Details
- ISSN :
- 15746968 and 03781097
- Volume :
- 256
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Letters
- Accession number :
- edsair.doi.dedup.....072cd024af4d1f65b6e9250b1a4be843