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The New Therapeutic Concept of Using a Rho Kinase Inhibitor for the Treatment of Corneal Endothelial Dysfunction
- Source :
- Cornea. 30:S54-S59
- Publication Year :
- 2011
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2011.
-
Abstract
- Corneal endothelial cells (CECs) show poor regenerative ability in humans, and noncompensatory damage of CECs causes irreversible corneal haziness in cases of bullous keratopathy. Although corneal transplantations provide considerable clinical benefits, allograft rejection, primary graft failure, and the shortage of donor corneas are problems that still need to be overcome. The establishment of new treatment therapies is the key to solving these problems, and we have attempted to establish a new clinical intervention for corneal endothelial dysfunction. We previously demonstrated that the inhibition of Rho/Rho kinase (ROCK) signaling by Y-27632, a specific ROCK inhibitor, promoted cell adhesion, inhibited apoptosis, and enhanced cell proliferation in cultured primate CECs. These results raise the possibility that the ROCK inhibitor might serve as a new tool for establishing an effective culture method for newly emerging cultivated CEC transplantation therapies. Moreover, because Y-27632 enhances cell proliferation in vitro, we hypothesized that the use of a ROCK inhibitor could be a new pharmacological intervention for the treatment of corneal endothelial dysfunction. We demonstrated that the topical instillation of a ROCK inhibitor promotes corneal endothelium wound healing in an animal model. This indicates that use of a ROCK inhibitor is a less invasive and novel therapy that should prove promising for the treatment of corneal endothelial dysfunction.
- Subjects :
- Corneal endothelium
Corneal Diseases
Animals
Medicine
Endothelial dysfunction
Cell adhesion
Protein Kinase Inhibitors
Rho-associated protein kinase
Cell Proliferation
Wound Healing
rho-Associated Kinases
business.industry
Endothelium, Corneal
medicine.disease
eye diseases
Transplantation
Macaca fascicularis
Ophthalmology
Rho kinase inhibitor
Models, Animal
Bullous keratopathy
Cancer research
Rabbits
sense organs
Ophthalmic Solutions
Wound healing
business
Subjects
Details
- ISSN :
- 02773740
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Cornea
- Accession number :
- edsair.doi.dedup.....071b97c72c1aa2e3ce5b90458ff38c97