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Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation

Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation

Authors :
Yasuyuki Ozeki
Takuya Asai
Makiko Goto
Haruyo Yamanishi
Mariko Egawa
Masashi Miyai
Chika Katagiri
Kyoya Tokunaga
Junichi Hosoi
Shinya Iwanaga
Source :
Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
Publication Year :
2019
Publisher :
Springer Science and Business Media LLC, 2019.

Abstract

Epidermal keratinocyte (KC) differentiation, which involves the process from proliferation to cell death for shedding the outermost layer of skin, is crucial for the barrier function of skin. Therefore, in dermatology, it is important to elucidate the epidermal KC differentiation process to evaluate the symptom level of diseases and skin conditions. Previous dermatological studies used staining or labelling techniques for this purpose, but they have technological limitations for revealing the entire process of epidermal KC differentiation, especially when applied to humans. Here, we demonstrate label-free visualization of three-dimensional (3D) intracellular morphological changes of ex vivo human epidermis during epidermal KC differentiation using stimulated Raman scattering (SRS) microscopy. Specifically, we observed changes in nuclei during the initial enucleation process in which the nucleus is digested prior to flattening. Furthermore, we found holes left behind by improperly digested nuclei in the stratum corneum, suggesting abnormal differentiation. Our findings indicate the great potential of SRS microscopy for discrimination of the degree of epidermal KC differentiation.

Details

ISSN :
20452322
Volume :
9
Database :
OpenAIRE
Journal :
Scientific Reports
Accession number :
edsair.doi.dedup.....0700dc60d6e8539d8fb66baaf0d76e8e
Full Text :
https://doi.org/10.1038/s41598-019-49035-x