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Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation
Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Epidermal keratinocyte (KC) differentiation, which involves the process from proliferation to cell death for shedding the outermost layer of skin, is crucial for the barrier function of skin. Therefore, in dermatology, it is important to elucidate the epidermal KC differentiation process to evaluate the symptom level of diseases and skin conditions. Previous dermatological studies used staining or labelling techniques for this purpose, but they have technological limitations for revealing the entire process of epidermal KC differentiation, especially when applied to humans. Here, we demonstrate label-free visualization of three-dimensional (3D) intracellular morphological changes of ex vivo human epidermis during epidermal KC differentiation using stimulated Raman scattering (SRS) microscopy. Specifically, we observed changes in nuclei during the initial enucleation process in which the nucleus is digested prior to flattening. Furthermore, we found holes left behind by improperly digested nuclei in the stratum corneum, suggesting abnormal differentiation. Our findings indicate the great potential of SRS microscopy for discrimination of the degree of epidermal KC differentiation.
- Subjects :
- Adult
Keratinocytes
0301 basic medicine
Cell biology
Programmed cell death
Science
Intracellular Space
Spectrum Analysis, Raman
Article
03 medical and health sciences
0302 clinical medicine
Stratum corneum
medicine
Humans
Barrier function
Aged
Organelles
Cell Nucleus
Microscopy
Multidisciplinary
integumentary system
Epidermis (botany)
Chemistry
Imaging and sensing
Cell Differentiation
Middle Aged
Staining
030104 developmental biology
medicine.anatomical_structure
Raman spectroscopy
Medicine
Female
Epidermis
Nucleus
030217 neurology & neurosurgery
Ex vivo
Intracellular
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....0700dc60d6e8539d8fb66baaf0d76e8e
- Full Text :
- https://doi.org/10.1038/s41598-019-49035-x