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Regulation of connexin gene expression during skeletal muscle regeneration in the adult rat
- Publication Year :
- 2009
-
Abstract
- In the adult skeletal muscle, various kinds of trauma promote proliferation of satellite cells that differentiate into myoblasts forming new myofibers or to repair the damaged one. The aim of present work was to perform a comparative spatial and temporal analysis of connexin (Cx) 37, Cx39, Cx40, Cx43, and Cx45 expression in the adult regenerating skeletal muscle in response to crush injury. Within 24 h from injury, Cx37 expression was upregulated in the endothelial cells of blood vessels, and, 5 days after injury, Cx37-expressing cells were found inside the area of lesion and formed clusters generating new blood vessels with endothelial cells expressing Cx37. Three days after injury, Cx39 mRNA was selectively expressed in myogenin-positive cells, forming rows of closely apposed cell nuclei fusing in myotubes. Cx40 mRNA-labeled cells were observed within 24 h from injury in the endothelium of blood vessels, and, 5 days after lesion, Cx40-labeled cells were found inside the area of lesion-forming rows of myogenin-positive, closely apposed cells coexpressing Cx39. Within 24 h from lesion, both Cx43 and Cx45 mRNAs were upregulated in individual cells, and some of them were positive for M-cadherin. Three days after injury, a large number of both Cx43 and Cx45 mRNA-labeled and myogenin-positive cells were found inside the area of lesion. Taken together, these results show that at least four Cxs, out of five expressed in regenerating skeletal muscle, can be differentially involved in communication of myogenic cells during the process of cell proliferation, aggregation, and fusion to form new myotubes or to repair damaged myofibers.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Time Factors
Physiology
Muscle Fibers, Skeletal
Connexin
Neovascularization, Physiologic
connexin 45
Biology
Connexins
connexin 43
Cell Fusion
connexin 40
Muscle regeneration
Gene expression
medicine
Connexin 30
Myocyte
Animals
Regeneration
RNA, Messenger
Rats, Wistar
Muscle, Skeletal
In Situ Hybridization
Cell Aggregation
Cell Proliferation
Myogenic cells
connexin 39
Regeneration (biology)
Skeletal muscle
Endothelial Cells
Cell Biology
connexin 37
biology.organism_classification
Constriction
Immunohistochemistry
Cell biology
Rats
medicine.anatomical_structure
Gene Expression Regulation
myogenic cell
Satellite (biology)
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....06fa4f7164105db191b8dd19a10c9259