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Micro- and Nanopatterned Topographical Cues for Regulating Macrophage Cell Shape and Phenotype
- Source :
- ResearcherID, ACS applied materials & interfaces, vol 7, iss 51
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Abstract
- Controlling the interactions between macrophages and biomaterials is critical for modulating the response to implants. While it has long been thought that biomaterial surface chemistry regulates the immune response, recent studies have suggested that material geometry may in fact dominate. Our previous work demonstrated that elongation of macrophages regulates their polarization towards a pro-healing phenotype. In this work, we elucidate how surface topology might be leveraged to alter macrophage cell morphology and polarization state. Using a deep etch technique, we fabricated titanium surfaces containing micro and nano-patterned grooves, which have been previously shown to promote cell elongation. Morphology, phenotypic markers, and cytokine secretion of murine bone marrow derived macrophages on different groove widths were analyzed. The results suggest that micro and nano-patterned grooves influenced macrophage elongation, which peaked on substrates with 400-500 nm wide grooves. Surface grooves did not affect inflammatory activation, but drove macrophages towards an anti-inflammatory, pro-healing phenotype. While secretion of TNF-alpha remained low in macrophages across all conditions, macrophages secreted significantly higher levels of anti-inflammatory cytokine, IL-10, on intermediate groove widths compared to cells on other Ti surfaces. Our findings highlight the potential of using surface topography to regulate macrophage function, and thus control the wound healing and tissue repair response to biomaterials.
- Subjects :
- Materials science
Biocompatibility
Surface Properties
medicine.medical_treatment
Cells
macrophage polarization
Macrophage polarization
Nanotechnology
Inbred C57BL
Article
Mice
biocompatibility
Engineering
topography
groove
medicine
Macrophage
Animals
General Materials Science
Secretion
titanium
Nanoscience & Nanotechnology
Cell Shape
Cells, Cultured
Cultured
Inflammatory and immune system
Macrophages
Biomaterial
Cell biology
Mice, Inbred C57BL
Cytokine
Phenotype
Chemical Sciences
Cytokines
Cytokine secretion
Wound healing
Biomarkers
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- ResearcherID, ACS applied materials & interfaces, vol 7, iss 51
- Accession number :
- edsair.doi.dedup.....06d7a25100c5e1f68091f35d2bb760ff