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Sodium para-aminosalicylate protected cultured basal ganglia astrocytes from manganese-induced DNA damages and alteration of amino acid neurotransmitter levels
- Source :
- The Journal of Toxicological Sciences. 41:573-581
- Publication Year :
- 2016
- Publisher :
- Japanese Society of Toxicology, 2016.
-
Abstract
- Sodium para-aminosalicylate (PAS-Na) was first applied successfully in clinical treatment of two manganism patients with good prognosis. However, the mechanism of how PAS-Na protects against Mn-induced neurotoxicity is still elusive. The current study was conducted to explore the effects of PAS-Na on Mn-induced basal ganglia astrocyte injury, and the involvement of amino acid neurotransmitter in vitro. Basal ganglia astrocytes were exposed to 500 μM manganese chloride (MnCl2) for 24 hr, following by 50, 150, or 450 μM PAS-Na treatment for another 24 hr. MnCl2 significantly decreased viability of astrocytes and induced DNA damages via increasing the percentage of tail DNA and Olive tail moment of DNA. Moreover, Mn interrupted amino acid neurotransmitters by decreasing Gln levels and increasing Glu, Gly levels. In contrast, PAS-Na treatment reversed the aforementioned Mn-induced toxic effects on basal ganglia astrocytes. Taken together, our results demonstrated that excessive Mn exposure may induce toxic effects on basal ganglia astrocytes, while PAS-Na could protect basal ganglia astrocytes from Mn-induced neurotoxicity.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Glutamine
Sodium
Glycine
Glutamic Acid
chemistry.chemical_element
Biology
Protective Agents
Toxicology
Basal Ganglia
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Chlorides
Internal medicine
Basal ganglia
medicine
Manganism
Animals
Cells, Cultured
chemistry.chemical_classification
Dose-Response Relationship, Drug
Manganese Poisoning
Neurotoxicity
medicine.disease
Aminosalicylic Acid
Amino acid
Dose–response relationship
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Animals, Newborn
Manganese Compounds
chemistry
Biochemistry
Cytoprotection
Astrocytes
Amino acid neurotransmitter
030217 neurology & neurosurgery
DNA Damage
Astrocyte
Subjects
Details
- ISSN :
- 18803989 and 03881350
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- The Journal of Toxicological Sciences
- Accession number :
- edsair.doi.dedup.....06cd4712e712b4cd7c6c554be9ce26ed