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Implantation of VEGF‐functionalized cell‐free vascular grafts: regenerative and immunological response
- Source :
- The FASEB Journal. 33:5089-5100
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Recently, our group demonstrated that immobilized VEGF can capture flowing endothelial cells (ECs) from the blood in vitro and promote endothelialization and patency of acellular tissue-engineered vessels (A-TEVs) into the arterial system of an ovine animal model. Here, we demonstrate implantability of submillimeter diameter heparin and VEGF-decorated A-TEVs in a mouse model and discuss the cellular and immunologic response. At 1 mo postimplantation, the graft lumen was fully endothelialized, as shown by expression of EC markers such as CD144, eNOS, CD31, and VEGFR2. Interestingly, the same cells coexpressed leukocyte/macrophage (Mϕ) markers CD14, CD16, VEGFR1, CD38, and EGR2. Notably, there was a stark difference in the cellular makeup between grafts containing VEGF and those containing heparin alone. In VEGF-containing grafts, infiltrating monocytes (MCs) converted into anti-inflammatory M2-Mϕs, and the grafts developed well-demarcated luminal and medial layers resembling those of native arteries. In contrast, in grafts containing only heparin, MCs converted primarily into M1-Mϕs, and the endothelial and smooth muscle layers were not well defined. Our results indicate that VEGF may play an important role in regulating A-TEV patency and regeneration, possibly by regulating the inflammatory response to the implants.-Smith, R. J., Jr., Yi, T., Nasiri, B., Breuer, C. K., Andreadis, S. T. Implantation of VEGF-functionalized cell-free vascular grafts: regenerative and immunological response.
- Subjects :
- Vascular Endothelial Growth Factor A
0301 basic medicine
CD31
Pathology
medicine.medical_specialty
CD14
Lumen (anatomy)
Inflammation
CD16
Biochemistry
Monocytes
Mice
03 medical and health sciences
0302 clinical medicine
Enos
Genetics
medicine
Animals
Endothelium
Molecular Biology
biology
Chemistry
Research
Macrophages
Heparin
biology.organism_classification
Immunohistochemistry
In vitro
Mice, Inbred C57BL
030104 developmental biology
Female
medicine.symptom
030217 neurology & neurosurgery
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 15306860 and 08926638
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- The FASEB Journal
- Accession number :
- edsair.doi.dedup.....06c5277b526fc54659862af30166bd42