Supplementary Methods, Figures 1 - 13 from DDB2 Suppresses Epithelial-to-Mesenchymal Transition in Colon Cancer

PDF file - 13093K, Supplementary Fig.1: Loss of DDB2 expression in human colon cancer. Supplementary Fig.2: Low magnification IHC data showing reduced expression of DDB2 in high grade colon cancer samples. Supplementary Fig.3: Depletion of DDB2 increases invasivness of colon cancer cells. Supplementary Fig.4: Polyclonal DDB2 knockdown colon cancer cells exhibit increased tumorigenicity Supplementary Fig.5: Polyclonal DDB2 knockdown colon cancer cells exhibit EMT Supplementary Fig.6: DDB2 knockdown colon cancer cells generate aggressive tumors in nude mice Supplementary Fig.7: Low magnification picture showing EMT in xenografted tumors Supplementary Fig.8: DDB2 expression blocks EMT induced by hypoxia and TGF-beta Supplementary Fig.9: Chromatin-IP using T7-antibody demonstrating interaction of T7-DDB2 with the VEGF, Snail and Zeb1 promoters. Supplementary Fig.10: Transcription factor binding sites in the promoter fragments bound by DDB2 Supplementary Fig.11: Western blot analyses for the DDB2-associated proteins and XPC in DDB2-depleted cells. Supplementary Fig.12: Experimental metastasis assay with polyclonal DDB2 knockdown cells. Supplementary Fig.13: Expression of DDB2 does not inhibit proliferation in SW620 cells.