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RNA Recognition Motif 2 of Yeast Pab1p Is Required for Its Functional Interaction with Eukaryotic Translation Initiation Factor 4G
- Publication Year :
- 1998
- Publisher :
- American Society for Microbiology, 1998.
-
Abstract
- The eukaryotic mRNA 3′ poly(A) tail and its associated poly(A)-binding protein (Pab1p) are important regulators of gene expression. One role for this complex in the yeast Saccharomyces cerevisiae is in translation initiation through an interaction with a 115-amino-acid region of the translation initiation factor eIF4G. The eIF4G-interacting domain of Pab1p was mapped to its second RNA recognition motif (RRM2) in an in vitro binding assay. Moreover, RRM2 of Pab1p was required for poly(A) tail-dependent translation in yeast extracts. An analysis of a site-directed Pab1p mutation which bound to eIF4G but did not stimulate translation of uncapped, polyadenylated mRNA suggested additional Pab1p-dependent events during translation initiation. These results support the model that the association of RRM2 of yeast Pab1p with eIF4G is a prerequisite for the poly(A) tail to stimulate the translation of mRNA in vitro.
- Subjects :
- Five prime untranslated region
EIF4G
Gene Expression
RNA-Binding Proteins
RNA, Fungal
Cell Biology
Saccharomyces cerevisiae
Biology
Molecular biology
Poly(A)-Binding Proteins
Eukaryotic translation initiation factor 4 gamma
Cell biology
Internal ribosome entry site
chemistry.chemical_compound
Eukaryotic translation
chemistry
Peptide Initiation Factors
Eukaryotic initiation factor
Gene Expression Regulation, Fungal
Protein Biosynthesis
Poly(A)-binding protein
biology.protein
Initiation factor
Eukaryotic Initiation Factor-4G
Molecular Biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....06a628b5ebe63ed614c5d85aab592e1d