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Upregulation of microRNA‐9‐5p inhibits apoptosis of chondrocytes through downregulating Tnc in mice with osteoarthritis following tibial plateau fracture
- Source :
- Journal of Cellular Physiology. 234:23326-23336
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Osteoarthritis (OA) is a common degenerative joint disease which is typically progressed with age, affecting smaller joints of hands, lower limbs, and the vertebral column. It has been reported that microRNAs could regulate the biological processes of OA. Therefore, the purpose of this study was to elucidate miR-9-5p's role in regulating cartilage remodeling of OA mice following tibial plateau fracture (TPF) through regulation of tenascin C (Tnc). Initially, we determined the expression of miR-9-5p and Tnc in mice with OA and then testified their relationship. The results displayed a high expression of Tnc, but a poor expression of miR-9-5p with high methylation in OA. Tnc was confirmed to be a target gene of miR-9-5p. Moreover, based on gain- and loss-function experiments, an increase of miR-9-5p and loss of Tnc had the potential to inhibit cell apoptosis, while facilitating cell proliferation, migration, invasion, and cartilage remodeling of mice with OA following TPF. This was further demonstrated by a higher expression of type II collagen, lower type X collagen, and protogenin expression. Subsequently, downregulation of miR-9-5p aggravated the pathological changes of mice, illustrated by an increase in the Mankin score. In conclusion, the present study proved that overexpression of miR-9-5p suppressed chondrocytes apoptosis and promoted cartilage remodeling through downregulating of Tnc in mice with OA.
- Subjects :
- 0301 basic medicine
Knee Joint
Physiology
Clinical Biochemistry
Type II collagen
Down-Regulation
Apoptosis
Osteoarthritis
Mice
03 medical and health sciences
Chondrocytes
0302 clinical medicine
Downregulation and upregulation
microRNA
medicine
Animals
biology
Cell growth
Chemistry
Cartilage
Tenascin C
Tenascin
Cell Biology
musculoskeletal system
medicine.disease
Up-Regulation
Mice, Inbred C57BL
Tibial Fractures
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
030220 oncology & carcinogenesis
Cancer research
biology.protein
Female
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 234
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....069128dbe8526c489667298359050e5c