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Long-Term Anti-Bacterial Immunity against Systemic Infection by Salmonella enterica Serovar Typhimurium Elicited by a GMMA-Based Vaccine
- Source :
- Vaccines, Volume 9, Issue 5, Vaccines, Vol 9, Iss 495, p 495 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Salmonella Typhimurium (STm) represents the most prevalent cause of invasive non-typhoidal Salmonella (iNTS) disease, and currently no licensed vaccine is available. In this work we characterized the long-term anti-bacterial immunity elicited by a STm vaccine based on Generalized Modules of Membrane Antigens (GMMA) delivering O:4,5 antigen, using a murine model of systemic infection. Subcutaneous immunization of mice with STmGMMA/Alhydrogel elicited rapid, high, and persistent antigen-specific serum IgG and IgM responses. The serum was bactericidal in vitro. O:4,5-specific IgG were also detected in fecal samples after immunization and positively correlated with IgG observed in intestinal washes. Long-lived plasma cells and O:4,5-specific memory B cells were detected in spleen and bone marrow. After systemic STm challenge, a significant reduction of bacterial load in blood, spleen, and liver, as well as a reduction of circulating neutrophils and G-CSF glycoprotein was observed in STmGMMA/Alhydrogel immunized mice compared to untreated animals. Taken together, these data support the development of a GMMA-based vaccine for prevention of iNTS disease.
- Subjects :
- 0301 basic medicine
Serotype
Salmonella
salmonellosis
Immunology
Spleen
medicine.disease_cause
Article
Microbiology
03 medical and health sciences
0302 clinical medicine
systemic infection
Antigen
Immunity
Drug Discovery
medicine
Pharmacology (medical)
030212 general & internal medicine
GMMA vaccine
cellular immune response
Pharmacology
Salmonella Typhimurium
biology
business.industry
antibody response
biology.organism_classification
030104 developmental biology
Infectious Diseases
medicine.anatomical_structure
Immunization
Salmonella enterica
Medicine
Bone marrow
business
Antibody response
Cellular immune response
Salmonellosis
Systemic infection
Subjects
Details
- Language :
- English
- ISSN :
- 2076393X
- Volume :
- 9
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Vaccines
- Accession number :
- edsair.doi.dedup.....068f306f63b1ac30aac42c472466a800