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Salinomycin and Sulforaphane Exerted Synergistic Antiproliferative and Proapoptotic Effects on Colorectal Cancer Cells by Inhibiting the PI3K/Akt Signaling Pathway in vitro and in vivo
- Source :
- OncoTargets and therapy
- Publication Year :
- 2020
- Publisher :
- Informa UK Limited, 2020.
-
Abstract
- Fang Liu,1,2,* Rong-Bin Lv,3,* Yan Liu,4 Qian Hao,1 Shu-Jie Liu,5 Yuan-Yuan Zheng,2 Cui Li,2 Cheng Zhu,1 Min Wang1,6 1Department of Geriatric Gastroenterology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, People’s Republic of China; 2Department of Gastroenterology, Taian City Central Hospital, Taian, Shandong 271000, People’s Republic of China; 3Department of PET/CT, Taian City Central Hospital, Taian, Shandong 271000, People’s Republic of China; 4Department of Emergency, Dongping Hospital Affiliated to Shandong First Medical University, Taian, Shandong 271500, People’s Republic of China; 5Department of Obstetrics and Gynecology, Zibo Spring Hospital Co., Ltd, Zibo, Shandong 255022, People’s Republic of China; 6Department of General Practice, Qilu Hospital of Shandong University, Jinan, Shandong 250012, People’s Republic of China*These authors contributed equally to this workCorrespondence: Min WangDepartment of Geriatric Gastroenterology, Qilu Hospital of Shandong University, 107 Wenhuaxi Road, Jinan, Shandong 250012, People’s Republic of ChinaTel +86-531-82166601Email doctorminmin@163.comBackground: Both salinomycin (SAL) and sulforaphane (SFN) exert their antitumorigenic effects in various types of cancer We investigated whether combining salinomycin (SAL, an antibiotic ionophore) with sulforaphane (SFN, a phytochemical) exerted synergistic antiproliferative and proapoptotic activities in colorectal cancer (CRC) cells in vitro and in vivo by evaluating the proliferative and apoptotic responses of two CRC cell lines.Materials and Methods: The combination index (CI) was calculated using the Chou-Talalay method, and the effects of the synergistic combination (CI< 1) of lower doses of SAL and SFN were selected for further studies. Anti-tumor effect of the combination of SAL and SFN was tested both in vitro and in vivo.Results: Cotreatment effectively inhibited proliferation, migration and invasion and enhanced apoptosis. The xenograft model also showed similar results. Furthermore, we evaluated the molecular mechanism behind SAL- and SFN-mediated CRC cell apoptosis. The combination treatment induced apoptosis in Caco-2 and CX-1 cells by inhibiting the PI3K/Akt pathway, which increased the expression of the tumor suppressor protein p53. The treatment also decreased the expression of the survival protein Bcl-2 and increased the expression of the proapoptotic protein Bax, which increased the Bax/Bcl-2 ratio, as well as enhanced poly ADP-ribose polymerase (PARP) cleavage. Upon inhibiting the PI3K/Akt pathway with LY294002 prior to cotreatment, we detected enhanced PARP cleavage compared to that in the cotreatment only group.Conclusion: We investigated whether the combination of SAL and SFN had antiproliferative and proapoptotic effects in CRC cells both in vitro and in vivo. Cotreatment also significantly decreased migration and invasion compared to that of the control and SAL or SFN monotherapies. This novel combination of SAL and SFN might provide a potential strategy to treat CRC.Keywords: salinomycin, sulforaphane, colorectal cancer, synergism, apoptosis, PI3K/Akt pathway
- Subjects :
- 0301 basic medicine
Poly ADP ribose polymerase
sulforaphane
colorectal cancer
OncoTargets and Therapy
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
In vivo
synergism
parasitic diseases
Pharmacology (medical)
LY294002
Salinomycin
PI3K/AKT/mTOR pathway
Original Research
apoptosis
In vitro
030104 developmental biology
Oncology
chemistry
PI3K/Akt pathway
Apoptosis
030220 oncology & carcinogenesis
salinomycin
Cancer research
Sulforaphane
Subjects
Details
- ISSN :
- 11786930
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- OncoTargets and Therapy
- Accession number :
- edsair.doi.dedup.....068972cfb1b6248482fd78e92ce6dbfc