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Sylimarin as hepatic protector and immunomodulator in Nile tilapia during Streptococcus agalactiae infection
- Source :
- Fishshellfish immunology. 82
- Publication Year :
- 2018
-
Abstract
- This study investigated the use of silymarin, an extract obtained from the milk thistle (Silybum marianum) and its effects as a possible hepatoprotector in Nile tilapia (Oreochromis niloticus). Silymarin was used as feed additive to the diet at a concentration of 0.1% (1 kg per ton of dry ration) with the commercial product named Di-Heptarine S® (16% silymarin phosphatide). A total of 90 juvenile tilapia with approximately 45 days old and mean weight of 0.72 ± 0.04 g were distributed in two groups, one fed with a diet with the hepatoprotector and the other without the additive. At the end of the assay (55 days after feeding), samples of blood were collected for hematological, immunological, histological (liver, spleen and intestine) and enzymatic analysis such as superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). After 55 days all fish were challenged with Streptococcus agalactiae serotype Ib to verify the sylimarin effects on the immunological parameters and its protection effect while challenged. During the challenge period another biological material sample was collected for hematological, immunological and histopathological analysis (liver, spleen and intestine). Before the challenge, an increase on the count of thrombocyte was found in the supplemented fish. In the liver, dilation of the sinusoids was observed in unsupplemented fish while supplemented fish the alteration was less severe. No significant alteration was found in SOD, CAT and GST between the groups. Histological changes after the challenge were provoked by bacterial toxins as a result of inflammatory processes. Periacinar degeneration was less intense in unsupplemented fish when compared to supplemented fish. On the other hand, eosinophilic and lymphocytic infiltrate did occur in unsupplemented fish differently from supplemented fish which did not show the alteration. The survival was 28% higher in silymarin supplemented fish when compared to unsupplemented fish that presented no survival. Silymarin supplementation in the diet provided a hepatoprotective and immunomodulatory effect on Nile tilapia.
- Subjects :
- 0301 basic medicine
food.ingredient
Feed additive
Spleen
Aquatic Science
Protective Agents
Silybum marianum
Streptococcus agalactiae
Superoxide dismutase
03 medical and health sciences
Nile tilapia
Fish Diseases
food
Animal science
Streptococcal Infections
medicine
Environmental Chemistry
Animals
Immunologic Factors
biology
Milk Thistle
Tilapia
04 agricultural and veterinary sciences
General Medicine
Cichlids
biology.organism_classification
Animal Feed
Diet
Oreochromis
030104 developmental biology
medicine.anatomical_structure
Liver
Dietary Supplements
040102 fisheries
biology.protein
0401 agriculture, forestry, and fisheries
Silymarin
Subjects
Details
- ISSN :
- 10959947
- Volume :
- 82
- Database :
- OpenAIRE
- Journal :
- Fishshellfish immunology
- Accession number :
- edsair.doi.dedup.....0680a0145eeb7ab8a684e36c9e7b7ad4