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Identification and molecular mapping of a Fusarium wilt resistant gene in upland cotton
- Source :
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 119(4)
- Publication Year :
- 2009
-
Abstract
- Fusarium wilt (FW) is one of the most economically damaging cotton diseases worldwide, causing yellowing, wilting, defoliation, vascular tissue damage and ultimately death. Identification of molecular markers linked to FW genes is vital to incorporate resistance into elite cotton cultivars. An intraspecific F2 in Gossypium hirsutum L. was developed by crossing with a highly resistant cultivar Zhongmiansuo 35 (ZMS35) and a susceptible cultivar Junmian 1 to screen simple sequence repeats (SSRs) closely linked to the FW resistance gene. FW was identified in F2:3 families by evaluating seedling leaf symptoms and vascular tissue damage at plant maturity under natural field infection conditions over 2 years. The results showed that FW resistance segregated in a 3:1 ratio as a simple monogenic trait in F2:3 families. Molecular mapping identified a FW resistance gene closely linked with the SSR marker JESPR304−280 in chromosome D3(c17). We proposed to name this gene FW R . A composite interval mapping method detected four QTLs for FW resistance in Chr.A7(c7), D1(c15), D9(c23) and D3, respectively. Among them, one major QTL (LOD > 20) was tagged near marker JESPR304 within an interval of 0.06–0.2 cM, and explained over 52.5–60.9% of the total phenotypic variance. The data confirmed the existence of a major gene in Chr.D3. This is the first report of molecular mapping of a major gene contributing FW resistance in cotton. The present research therefore provides an opportunity to understand the genetic control of resistance to FW and conduct molecular marker-assisted selection breeding to develop FW resistant cultivars.
- Subjects :
- medicine.medical_specialty
Quantitative Trait Loci
Inheritance Patterns
Quantitative trait locus
Biology
Genes, Plant
Gene mapping
Fusarium
Molecular genetics
Botany
Genetics
medicine
Cultivar
Plant Diseases
Gossypium
food and beverages
Wilting
Chromosome Mapping
General Medicine
Major gene
Fusarium wilt
Immunity, Innate
Horticulture
Genetic marker
Agronomy and Crop Science
Biotechnology
Subjects
Details
- ISSN :
- 14322242
- Volume :
- 119
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
- Accession number :
- edsair.doi.dedup.....065e4b52c42bdb053ffda510b2abedc7