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Activation of calcium-sensing receptor-mediated autophagy in angiotensinII-induced cardiac fibrosis in vitro
- Source :
- Biochemical and Biophysical Research Communications. 497:571-576
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Cardiac fibrosis is one of the primary mechanisms of ventricular remodeling, and there is no effective method for reversal. Activation of calcium sensing receptor (CaSR) has been reported to be involved in the development of myocardial fibrosis, but the molecular mechanism for CaSR activation has not yet been clarified and needs to be further explored. Here, we found that AngII induces cardiac fibroblast proliferation and phenotypic transformation in a dose-dependent manner with increased CaSR and autophagy related protein (Beclin1, LC3B) expression. CaSR activation results in intracellular calcium release, MEK1/2 pathway phosphorylation, autophagy activation and collagen formation induced by AngII in cardiac fibroblasts. However, pretreating the cells with Calhex231, PD98059 or 3-MA partially blocked AngII-induced cardiac fibrosis. Our data indicate that the activation of CaSR-mediated MEK/ERK and autophagic pathways is involved in AngII-induced cardiac fibrosis in vitro.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
MAP Kinase Signaling System
Cardiac fibrosis
Biophysics
Biochemistry
Calcium in biology
03 medical and health sciences
Fibrosis
Autophagy
medicine
Animals
Rats, Wistar
Molecular Biology
Cells, Cultured
Chemistry
Angiotensin II
Myocardium
Cell Biology
Fibroblasts
medicine.disease
Cell biology
030104 developmental biology
cardiovascular system
Myocardial fibrosis
Collagen
Calcium-sensing receptor
Receptors, Calcium-Sensing
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 497
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....0654c44108e664c790411edbc9c62f9a