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Plasmid-determined cloacin DF13-susceptibility inEnterobacter cloacae andKlebsiella edwardsii; identification of the cloacin DF13/aerobactin outer membrane receptor proteins
- Source :
- Antonie van Leeuwenhoek. 51:203-218
- Publication Year :
- 1985
- Publisher :
- Springer Science and Business Media LLC, 1985.
-
Abstract
- Both Enterobacter cloacae H478 and Klebsiella edwardsii S15 were shown to harbour a relatively large conjugative plasmid that coded for cloacin DF13-susceptibility and the production and uptake of a hydroxamate iron chelator, most probably aerobactin. Protein-blotting experiments with antiserum raised against the purified cloacin DF13/aerobactin receptor protein from Escherichia coli (Co1V-K30) revealed that the corresponding outer membrane receptor proteins of Ent. cloacae H478 and K. edwardsii S15 had apparent mol wts of 85 000 and 76 000, respectively. E. coli transconjugants harbouring either the plasmid from Ent. cloacae H478 or K. edwardsii S15 expressed a cloacin DF13/aerobactin outer membrane receptor protein with a mol wt of 74 000. The receptor protein encoded by the Ent. cloacae and K. edwardsii plasmids were immunologically more related to each other than to the pCo1V-K30-encoded receptor protein.
- Subjects :
- Enterobacter
Cloacin
Hydroxamic Acids
Iron Chelating Agents
Microbiology
chemistry.chemical_compound
Plasmid
Enterobacteriaceae
Cell surface receptor
Klebsiella
Receptors, Immunologic
Molecular Biology
Antiserum
biology
General Medicine
biology.organism_classification
Molecular Weight
chemistry
Membrane protein
Conjugation, Genetic
Aerobactin
Bacterial outer membrane
Enterobacter cloacae
Bacterial Outer Membrane Proteins
Plasmids
Subjects
Details
- ISSN :
- 15729699 and 00036072
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Antonie van Leeuwenhoek
- Accession number :
- edsair.doi.dedup.....064b780326443af5348af60f4fe06ef7