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Structure of a novel phosphocholine-containing glycoglycerolipid from Mycoplasma fermentans
- Source :
- The Journal of biological chemistry. 269(52)
- Publication Year :
- 1994
-
Abstract
- Mycoplasma fermentans is thought to be a pathogen of rheumatoid arthritis or cofactor of AIDS. A novel phosphocholine-containing glycoglycerophospholipid named GGPL-I was isolated from a M. fermentans-infected human helper T-cell culture. It was revealed that GGPL-I is a lipid component of the M. fermentans and a major immunological determinant. The GGPL-I was purified by DEAE-Sephadex column chromatography and repeated Iatrobeads column chromatography. The purified glycophospholipid was subjected to structural characterization by thin-layer chromatography, Fourier-transform infrared spectrometry, liquid secondary ion mass spectrometry, and nuclear magnetic resonance spectroscopy. Its structure was determined to be as follows: 6'-O-phosphocholine-alpha-glucopyranosyl-(1'-3)-1,2-diacyl-sn-glycerol. This glycoglycerophospholipid is unique in containing phosphocholine, which is attached to C-6 of glucose. The stereospecific numbering (sn) of naturally occurring GGPL-I was determined through comparison with chemically synthesized compounds.
- Subjects :
- Glycerol
Magnetic Resonance Spectroscopy
Spectrophotometry, Infrared
Molecular Sequence Data
Mass spectrometry
Biochemistry
Cofactor
Mass Spectrometry
Cell Line
Choline
chemistry.chemical_compound
Column chromatography
Carbohydrate Conformation
Humans
Mycoplasma fermentans
Molecular Biology
Pathogen
Phosphocholine
Chromatography
biology
Chemistry
Cell Biology
Nuclear magnetic resonance spectroscopy
T-Lymphocytes, Helper-Inducer
biology.organism_classification
Glucose
Carbohydrate Sequence
biology.protein
Carbohydrate conformation
Glycolipids
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 269
- Issue :
- 52
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....064018722742c77bcbed0d5ac4d6cf3c