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Quantification of peripheral whole blood, cell-free plasma and exosome encapsulated mitochondrial DNA copy numbers in patients with atrial fibrillation
- Source :
- Journal of Biotechnology. 299:66-71
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Mitochondrial DNA (mtDNA) copy number changes have been associated with various diseases. Several studies showed that mtDNA content in peripheral blood was associated with oxidative stress and cardiovascular disease. Atrial fibrillation (AF) is one of the severe cardiovascular diseases. We aimed to determine the mtDNA copy numbers in peripheral blood, in cell-free plasma and in exosomes of AF patients and healthy controls. Peripheral blood was drawn from 60 AF patients and 72 healthy controls. DNA was isolated from EDTA blood and plasma. Exosomes were isolated from cell-free plasma and then exosome encapsulated DNA (exoDNA) was extracted. Quantitative-real-time PCR was performed with Human Mitochondrial DNA (mtDNA) Monitoring Primer Set. Statistical analysis of the data was performed. We found statistically significant difference in mtDNA copy numbers in DNA isolated from peripheral whole blood, cell-free plasma and exosome samples of controls’ (44.4 ± 18.0, 27.2 ± 30.1, 11.5 ± 8.7), and patients’ group (43.4 ± 13.6, 26.2 ± 26.4, 14.5 ± 12.3). However there was no significant difference in mtDNA copy number between the two study groups either in peripheral blood, in cell-free plasma and in exosomes, and even in different sexes and ages. We found the highest copy number of mtDNA in peripheral blood, followed by plasma and exosomes. We did not find differences between patients and controls, neither age nor gender had effect on the mtDNA copy number. According to our results the mtDNA copy numbers did not differ in AF patients.
- Subjects :
- Adult
Male
0106 biological sciences
0301 basic medicine
Mitochondrial DNA
DNA Copy Number Variations
Bioengineering
Biology
Exosomes
medicine.disease_cause
DNA, Mitochondrial
01 natural sciences
Applied Microbiology and Biotechnology
Exosome
Human mitochondrial genetics
Andrology
03 medical and health sciences
chemistry.chemical_compound
010608 biotechnology
Atrial Fibrillation
medicine
Humans
Elméleti orvostudományok
Aged
Whole blood
Orvostudományok
General Medicine
Middle Aged
Microvesicles
Mitochondria
030104 developmental biology
chemistry
Case-Control Studies
Female
Primer (molecular biology)
Cell-Free Nucleic Acids
Oxidative stress
DNA
Biotechnology
Subjects
Details
- ISSN :
- 01681656
- Volume :
- 299
- Database :
- OpenAIRE
- Journal :
- Journal of Biotechnology
- Accession number :
- edsair.doi.dedup.....063f60fdc534de5a60c3b73e6b23b645