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An immunochemical focus assay to quantify replication competent and defective viruses involved in murine acquired immunodeficiency syndrome
- Source :
- Journal of virological methods. 77(2)
- Publication Year :
- 1999
-
Abstract
- Murine acquired immunodeficiency syndrome is a lymphoproliferative disease with a concurrently developing immunodeficiency. The disease is induced after injection of supernatant of a chronically infected cell line that releases a mixture of two replication competent virus classes and a replication incompetent virus species responsible for pathogenicity. An immunochemical detection assay for virus infected foci on cell monolayers has been developed. This assay allows quantification of all three types of virus.
- Subjects :
- medicine.drug_class
viruses
Biology
Monoclonal antibody
Virus Replication
Defective virus
Virus
Cell Line
Immunoenzyme Techniques
Mice
Mink Cell Focus-Inducing Viruses
Murine Acquired Immunodeficiency Syndrome
Virology
Murine leukemia virus
medicine
Animals
Immunodeficiency
Virus classification
Ecotropism
Defective Viruses
medicine.disease
biology.organism_classification
Mice, Inbred C57BL
Female
Viral disease
Subjects
Details
- ISSN :
- 01660934
- Volume :
- 77
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of virological methods
- Accession number :
- edsair.doi.dedup.....063633acc25e80ba0f25f9b9a7c76c3c