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Sensitivity to Ultraviolet Light of Tobacco Mosaic Virus Modified by Cetyltrimethylammonium Bromide
- Source :
- Microbiology and Immunology. 25:1129-1138
- Publication Year :
- 1981
- Publisher :
- Wiley, 1981.
-
Abstract
- Cetyltrimethylammonium bromide (CTAB) modified tobacco mosaic virus (TMV) virions so that the intrinsic fluorescence changed, viral infectivity decreased, sensitivity to RNase or UV irradiation increased, and coat protein subunits were released by the addition of Triton X-100. The change in fluorescence emission at 320 nm shifted to 340 nm was observed at 100 micrograms of CTAB per ml. This represents a change in the tryptophan environment inside the virion. At a lower concentration of CTAB, intersubunit contact was weakened, resulting in the release of coat protein subunits and an increase in RNase sensitivity. The release of coat protein took place gradually and two relatively stable intermediates were observed. Increase in UV sensitivity was observed at a lower concentration of CTAB and formation of pyrimidine hydrate was involved in this inactivation. The nature of the minor structural change leading to UV inactivation is discussed.
- Subjects :
- Pyrimidine
Photochemistry
Ultraviolet Rays
RNase P
Immunology
Biology
Microbiology
chemistry.chemical_compound
Capsid
Ribonucleases
Bromide
Virology
Tobacco mosaic virus
Ultraviolet light
Virulence
Tryptophan
Fluorescence
Quaternary Ammonium Compounds
Tobacco Mosaic Virus
Spectrometry, Fluorescence
Biochemistry
chemistry
Cetrimonium Compounds
Biophysics
RNA, Viral
Hydrate
Subjects
Details
- ISSN :
- 03855600
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Microbiology and Immunology
- Accession number :
- edsair.doi.dedup.....06311e6c37f23a2b12624e6569ed4407