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Aqueous extract of Bruguiera gymnorrhiza leaves protects against dextran sulfate sodium induced ulcerative colitis in mice via suppressing NF-κB activation and modulating intestinal microbiota
- Source :
- Journal of ethnopharmacology. 251
- Publication Year :
- 2019
-
Abstract
- Ethnopharmacological relevance Ulcerative colitis (UC) is tightly associated with inflammation response and oxidative stress. As a folk medicine applied in treatment of diarrhea, Bruguiera gymnorrhiza also possesses anti-inflammatory and anti-oxidative activities, which indicated that B. gymnorrhiza may exert anti-colitis effect. Aim of the study To investigate effect and mechanism of B. gymnorrhiza on experimental UC. Materials and methods Aqueous extract of B. gymnorrhiza leaves (ABL) was used for investigation in the present study. Murine UC was established through access to 3% dextran sulfate sodium (DSS) for 7 days. Meanwhile, mice accepted treatment with ABL (25, 50, 100 mg/kg) or sulfasalazine (200 mg/kg) once daily. On the last day, disease activity index (DAI) including body weight loss, fecal character and degree of bloody diarrhea was evaluated, colon segments were obtained for length measurement and further analysis and feces were collected for intestinal microbiota analysis. Results ABL ameliorated DAI scores, colon length shortening and histopathological damage in DSS-induced colitis mice obviously. SOD activity, levels of MDA and GSH altered by colitis were restored remarkably after ABL treatment. ABL inhibited increases in levels of colonic COX-2, iNOS, TNF-α, IL-6, IL-1β, IL-4, IL-10 and IL-11 in colitis mice. Moreover, ABL prominently suppressed NF-κB p65 and IκB phosphorylation and down-regulated mRNA levels of COX-2, iNOS, TNF-α, IL-6 and IL-1β elevated by colitis. As shown in microbiota analysis, ABL modulated composition of intestinal microbiota of colitis mice. Conclusion ABL exhibited protective effect against DSS-induced ulcerative colitis through suppressing NF-κB activation and modulating intestinal microbiota.
- Subjects :
- Male
Colon
Anti-Inflammatory Agents
Nitric Oxide Synthase Type II
Inflammation
Pharmacology
medicine.disease_cause
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Sulfasalazine
Drug Discovery
medicine
Animals
Colitis
030304 developmental biology
0303 health sciences
Mice, Inbred BALB C
ABL
Plant Extracts
Dextran Sulfate
NF-kappa B
Glutathione
medicine.disease
Ulcerative colitis
Gastrointestinal Microbiome
Plant Leaves
Diarrhea
chemistry
Cyclooxygenase 2
030220 oncology & carcinogenesis
Cytokines
Rhizophoraceae
Colitis, Ulcerative
medicine.symptom
Oxidative stress
medicine.drug
Subjects
Details
- ISSN :
- 18727573
- Volume :
- 251
- Database :
- OpenAIRE
- Journal :
- Journal of ethnopharmacology
- Accession number :
- edsair.doi.dedup.....05fcdda8eb498fb9f65fc9be58f9d430