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Low dose radiation attenuates inflammation and promotes wound healing in a mouse burn model
- Source :
- Journal of Dermatological Science. 96:81-89
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Background Burn injuries are devastating traumas that functionally affect a variety of organ systems. As intensive inflammatory responses induced by burns can lead to multiple organ failures and impaired skin regeneration increases risk of infectious complex, multimodal therapeutic approaches are needed. Objectives To investigate the role of low dose radiation (LDR) treatment for regulation of excessive inflammation and wound healing after burn injury. Methods Mouse burn model was established by generating third-degree burn injury in dorsal skin and local LDR less than 100 mGy was delivered to the mice. After 3 or 12 days after burn injury, systemic inflammation in liver, lung, spleen, and kidney and skin wound healing were assessed. For investigation of molecular mechanisms, HaCaT keratinocytes were administrated with serum from mice with burn injury and alteration of viability and cornification biomarkers are assessed. Results In a mouse burn model, expression of proinflammatory cytokines, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, were downregulated by LDR in major organs and wound healing capacity was increased by LDR. In skin tissue, we observed the alleviation of reactive oxygen species generation and increased antioxidant gene expression by LDR. In addition, we found that treatment of serum from mice with burn injury and LDR increased proliferation and cornification in HaCaT cells through activation of focal adhesion kinase signaling pathway. Conclusion LDR could reduce proinflammatory signaling pathway and increase skin wound healing after burn injury. Therefore, the present study suggested LDR as a novel treatment for burn injury patients.
- Subjects :
- Keratinocytes
0301 basic medicine
Burn injury
Inflammation
Dermatology
Radiation Dosage
Systemic inflammation
Biochemistry
Cell Line
Proinflammatory cytokine
Mice
030207 dermatology & venereal diseases
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Molecular Biology
Skin
Wound Healing
integumentary system
business.industry
Interleukin
Mice, Inbred C57BL
Disease Models, Animal
HaCaT
030104 developmental biology
Cancer research
Cytokines
Female
Tumor necrosis factor alpha
medicine.symptom
Burns
Wound healing
business
Oxidation-Reduction
Signal Transduction
Subjects
Details
- ISSN :
- 09231811
- Volume :
- 96
- Database :
- OpenAIRE
- Journal :
- Journal of Dermatological Science
- Accession number :
- edsair.doi.dedup.....05e76d2f70eaf484558b901946e762da