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Evaluation of the toxicities of silver and silver sulfide nanoparticles against Gram‐positive and Gram‐negative bacteria
- Source :
- IET Nanobiotechnol
- Publication Year :
- 2019
- Publisher :
- The Institution of Engineering and Technology, 2019.
-
Abstract
- In this study, the endogenous lipid signalling molecules, N ‐myristoylethanolamine, were explored as a capping agent to synthesise stable silver nanoparticles (AgNPs) and Ag sulphide NPs (Ag(2) S NPs). Sulphidation of the AgNPs abolishes the surface plasmon resonance (SPR) maximum of AgNPs at 415 nm with concomitant changes in the SPR, indicating the formation of Ag(2) S NPs. Transmission electron microscopy revealed that the AgNPs and Ag(2) S NPs are spherical in shape with a size of 5–30 and 8–30 nm, respectively. AgNPs and Ag(2) S NPs exhibit antimicrobial activity against Gram‐positive and Gram‐negative bacteria. The minimum inhibitory concentrations (MIC) of 25 and 50 μM for AgNPs and Ag(2) S NPs, respectively, were determined from resazurin microtitre plate assay. At or above MIC, both AgNPs and Ag(2) S NPs decrease the cell viability through the mechanism of membrane damage and generation of excess reactive oxygen species.
- Subjects :
- inorganic chemicals
Gram-negative bacteria
Silver
Silver sulfide
Gram-positive bacteria
education
Nanoparticle
Metal Nanoparticles
02 engineering and technology
Microbial Sensitivity Tests
010402 general chemistry
Gram-Positive Bacteria
01 natural sciences
Silver nanoparticle
chemistry.chemical_compound
Microscopy, Electron, Transmission
mental disorders
Gram-Negative Bacteria
Electrical and Electronic Engineering
Surface plasmon resonance
health care economics and organizations
biology
technology, industry, and agriculture
Silver Compounds
Resazurin
Surface Plasmon Resonance
021001 nanoscience & nanotechnology
biology.organism_classification
0104 chemical sciences
Electronic, Optical and Magnetic Materials
chemistry
0210 nano-technology
Antibacterial activity
Biotechnology
Nuclear chemistry
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- IET Nanobiotechnol
- Accession number :
- edsair.doi.dedup.....05c610e135deaecb6f8bd7f46b36d117